Protein Info for PP_2057 in Pseudomonas putida KT2440

Annotation: Uncharacterized transporter YraO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details transmembrane" amino acids 24 to 43 (20 residues), see Phobius details amino acids 55 to 76 (22 residues), see Phobius details amino acids 95 to 119 (25 residues), see Phobius details amino acids 134 to 154 (21 residues), see Phobius details amino acids 174 to 196 (23 residues), see Phobius details amino acids 232 to 250 (19 residues), see Phobius details amino acids 255 to 273 (19 residues), see Phobius details amino acids 285 to 305 (21 residues), see Phobius details amino acids 325 to 343 (19 residues), see Phobius details amino acids 349 to 366 (18 residues), see Phobius details amino acids 379 to 398 (20 residues), see Phobius details amino acids 410 to 432 (23 residues), see Phobius details TIGR00784: citrate transporter" amino acids 1 to 432 (432 residues), 651.2 bits, see alignment E=4.9e-200 PF03553: Na_H_antiporter" amino acids 8 to 175 (168 residues), 34.2 bits, see alignment E=2.5e-12 PF03600: CitMHS" amino acids 14 to 378 (365 residues), 120.3 bits, see alignment E=1.5e-38 PF06808: DctM" amino acids 233 to 425 (193 residues), 26.5 bits, see alignment E=4.3e-10

Best Hits

Swiss-Prot: 66% identical to YRAO_BACSU: Uncharacterized transporter YraO (yraO) from Bacillus subtilis (strain 168)

KEGG orthology group: K03300, citrate-Mg2+:H+ or citrate-Ca2+:H+ symporter, CitMHS family (inferred from 99% identity to ppf:Pput_3683)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L78 at UniProt or InterPro

Protein Sequence (434 amino acids)

>PP_2057 Uncharacterized transporter YraO (Pseudomonas putida KT2440)
MLALLGLIMVVTFTYLIMSKRLSPIVALTIVPIVFALVGGFAPDLGKMMLDGLKMVAPSA
ALLLFAILFFGLMIDAGLFDPLIRKILKRVNGDPIKIAIGTALLSLLVALDGDGTTTYMI
TCAAMLPLYKRIGMNPMILATVSMLSLSIMSGMSPWGGPATRAIAALGLDATEYFIPMLP
TVIGGAAWVVFTAYLLGRAERRRIGNIALESGGGNCYIKEILGDNPHKRPRLAYVNLVLV
IAVMTALVLGLMHAAILFMIGFVAALMINYPQLDLQKERILAHSGNAMTVVLLVFAAGIF
AGIFSGTKMVDALAQTLVDWIPEAWSHWFPLVVALTSMPLTFVLSNDAYYFGVVPILANA
AAAYGIDPVEIARASVLGQPVHLMSPLVASTLLLVGMVDRDIGDFQKATVKWAVLTSLVI
TALALLTGALSFMV