Protein Info for PP_2048 in Pseudomonas putida KT2440

Annotation: putative acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 transmembrane" amino acids 515 to 532 (18 residues), see Phobius details PF12418: AcylCoA_DH_N" amino acids 3 to 25 (23 residues), 35.6 bits, see alignment (E = 1.9e-12) PF02771: Acyl-CoA_dh_N" amino acids 39 to 155 (117 residues), 49.9 bits, see alignment E=9.5e-17 PF02770: Acyl-CoA_dh_M" amino acids 160 to 264 (105 residues), 58.6 bits, see alignment E=1.4e-19 PF00441: Acyl-CoA_dh_1" amino acids 274 to 446 (173 residues), 73.6 bits, see alignment E=5e-24 PF12806: Acyl-CoA_dh_C" amino acids 460 to 585 (126 residues), 99.4 bits, see alignment E=4.1e-32

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2048)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L87 at UniProt or InterPro

Protein Sequence (591 amino acids)

>PP_2048 putative acyl-CoA dehydrogenase (Pseudomonas putida KT2440)
MNYQAPLRDMRFVLHELFDLSGHCQQLGIELDRDTLDGILEEAARFTGQIIAPLNRNSDE
QGCQWVGGEVTTPDGMRDAYRQYVEHGWASMTGPQAYGGQGLPQMASASFHEMLMAASLS
FRIYSGLTEGAVLALHRHGSEPLKQAYLSRMVSGEWAGTMCLTEPQAGTDLALLRTRAQP
EADGSYRITGSKIFISGGEQDLTDNIVHLVLARLPDAPAGVRGISLFLVPKLLPGGERNA
LGCGALEHKMGIKGASTCVMNFDGAQGWLVGEANQGLACMFTMMNDARFQVGLQGLGIAE
AAFQGGLAYARERLQSRSLAGPVAAEKPADPIIVHPDVRRMLLTQKTLTEGCRMLGIYTA
RQLDLEHAAKSAPARQAAARRAALLIPIVKAFFTDVGQEVASLGVQVYGGHGYIREWGME
QLMRDSRITQLYEGTNGIQALDLIRRKVLGDGGAELNLLIDEMAPHVRAANALGGLQAMA
TGVGQRLEEWRALSGHVVEACERDPQESGAVSVDFLAYSAYVLLAAMWLQAAVRAQATLA
TGSAETAFYRAKLDAATFYWRRILPRATAHREALLGGADCLMALPASDFAF