Protein Info for PP_2045 in Pseudomonas putida KT2440

Annotation: Metallo-beta-lactamase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 653 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00753: Lactamase_B" amino acids 119 to 212 (94 residues), 64.3 bits, see alignment E=2.3e-21 PF14863: Alkyl_sulf_dimr" amino acids 382 to 521 (140 residues), 220.5 bits, see alignment E=1.5e-69 PF14864: Alkyl_sulf_C" amino acids 532 to 653 (122 residues), 132.3 bits, see alignment E=1.8e-42

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_2045)

Predicted SEED Role

"alkyl sulfatase (EC 3.1.6.-)" (EC 3.1.6.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.6.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88L90 at UniProt or InterPro

Protein Sequence (653 amino acids)

>PP_2045 Metallo-beta-lactamase family protein (Pseudomonas putida KT2440)
MRFSLSMLALALLAPLPSLGATAAKDATAATLASNQQWLQRLPFEDRVDYETARRGLVER
FNGPVATADGKTVWNLAQYAFLEPAQSPATVNPSLWRIAQLNNIAGLFKVTEGIYQLRGL
DLANMTIVEGKDGLIVLDPLLAVETAKAGLELYFKHRPRKPVTTVIYTHPHVDHFGGVRG
VINEADVKAGKVQVIAPEGFFEHAIGENVLAGPAMKRRAQYMYGAPLPRGPRGQVDAGLG
KGVPANATVSLIAPTLEISQPLQRMTLSGVEVEFQLTPGTEAPAEMNIYFPALRALCMAE
NATHVQHNVLTLRGALVRDPKVWAHYLDQSLVRYGEQAEVVFAQHHWPTWGGAAIRDYLA
DQRDMYAFIDSQTLRLINQGQTPMEIAQTLASLPPRLASKWYSRDYYGSLSHNVRAVYQR
YMGFYDGNPANLDPLPPQAAGKRYVAAMGGADQVLTQARQAFAEGDYRWVAQLANHLVFA
EPDNTEARELQADALEQLGYQSENATWRNAYLSGAQELRSGVAAGAGSGGNADDMVRALT
PSLFFDYLGVRVNAYNAADSNLTINWRFSDLDEDYALTLRNGVLTHRDLARHAKADVEVI
MRKQTLDSIALKQTGFLKEATAGAIEIKGERVKFLQFMGGLEEADSRFNIVTP