Protein Info for PP_2035 in Pseudomonas putida KT2440

Annotation: benzoate transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 55 to 74 (20 residues), see Phobius details amino acids 80 to 98 (19 residues), see Phobius details amino acids 104 to 123 (20 residues), see Phobius details amino acids 134 to 155 (22 residues), see Phobius details amino acids 175 to 194 (20 residues), see Phobius details amino acids 214 to 236 (23 residues), see Phobius details amino acids 258 to 286 (29 residues), see Phobius details amino acids 298 to 323 (26 residues), see Phobius details amino acids 329 to 351 (23 residues), see Phobius details amino acids 361 to 390 (30 residues), see Phobius details TIGR00843: benzoate transporter" amino acids 12 to 392 (381 residues), 395.5 bits, see alignment E=1.3e-122 PF03594: BenE" amino acids 21 to 391 (371 residues), 433.4 bits, see alignment E=3.5e-134

Best Hits

Swiss-Prot: 43% identical to YDCO_ECOLI: Inner membrane protein YdcO (ydcO) from Escherichia coli (strain K12)

KEGG orthology group: K05782, benzoate membrane transport protein (inferred from 100% identity to ppf:Pput_3707)

Predicted SEED Role

"Benzoate transport protein" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LA0 at UniProt or InterPro

Protein Sequence (404 amino acids)

>PP_2035 benzoate transport protein (Pseudomonas putida KT2440)
MDAPAQSSATPLRLTDILHPVIAGLVSVIVNYGGTFILVFQAAKVAGLSPELTSSWVWAV
SIGVGLSGLALSWYSREPVITAWSTPAAAFLVTALASVPYSEAIGAYLLSAAGFVVLGLS
GCFERLVRVVPGGIAAGLLAGILLQFGIGAFASLSVDPSLAGLLIAAYVAFKRFTARYAV
VAILLLGLGYLLLQGQVGLTTLRLEFASPVFTAPTFSLNAALSVALPLFLITLTGQYMPG
MLVLRNDGFKTSANPIVAVTGMGSLLMAPFGSHAFNIAAITAAICTGKEAHENPDKRWVA
GIAAGVCYILVGVFGVTLASVFMALPGTFITTLAGLALLGTIGASLASALADARSRESAL
ITFLAAAANISLLGIGGAFWGLVIGLLAHVVLNGRLPGLRGASR