Protein Info for PP_2019 in Pseudomonas putida KT2440

Updated annotation (from data): adhesin-associated MmpL efflux pump
Rationale: Conserved cofit with a putative adhesin (PP_0765). The MmpL family is involved in efflux of lipids or siderophores (see IPR004869); we speculate that this exports a lipid or saccharide that is attached to the adhesin.
Original annotation: putative multidrug efflux transporter, RND family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 799 transmembrane" amino acids 29 to 46 (18 residues), see Phobius details amino acids 243 to 265 (23 residues), see Phobius details amino acids 273 to 294 (22 residues), see Phobius details amino acids 300 to 317 (18 residues), see Phobius details amino acids 344 to 367 (24 residues), see Phobius details amino acids 376 to 401 (26 residues), see Phobius details amino acids 430 to 449 (20 residues), see Phobius details amino acids 631 to 649 (19 residues), see Phobius details amino acids 656 to 677 (22 residues), see Phobius details amino acids 683 to 703 (21 residues), see Phobius details amino acids 730 to 750 (21 residues), see Phobius details amino acids 758 to 782 (25 residues), see Phobius details PF03176: MMPL" amino acids 484 to 781 (298 residues), 105 bits, see alignment E=2e-34

Best Hits

KEGG orthology group: K07003, (no description) (inferred from 100% identity to ppu:PP_2019)

Predicted SEED Role

"FIG005548: transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LB6 at UniProt or InterPro

Protein Sequence (799 amino acids)

>PP_2019 adhesin-associated MmpL efflux pump (Pseudomonas putida KT2440)
MTSRESITMHPHHQDKATLLERLIFNNRPVVIALCVLVSIFLFWQATQIRPSTSFEKMIP
LQHPFIEQMMEHRNDLANLGNTVRISVEAVNGDIFDKDYMETLRQIHDEVFYIPGVDRAG
LKSLWSPSVRWSEVTEEGFSGGEVIPNTYNGSQDSLDTLRDNVLKSGQVGRLVGNNFKSS
IVDVPLLESYPDPQDPGKQVKLDYQQFSHLLEEKIRDKFQAQNPNVKIHIVGFAKKVGDL
IDGLVMVAMFFGVALVITLVLLYWFTWCIRSTVAVLITTLVAVVWQLGLMHAVGFGLDPY
SMLVPFLIFAIGISHGVQKINGIALQSSDADNALTAARRTFRQLFLPGMIAILADAVGFI
TLLIIDIGVIRELAIGASIGVAVIVFTNLILLPVAISYVGISKKAIERSKKDATCEHPFW
RLLSNFASAKVAPVSVVLALLAFAGGLWYSQNLKIGDLDQGAPELRPDSRYNQDNNFIIS
NYSTSSDVLVIMVKTPSEKCSVHSTMAPIDELMWTMENTPGVQSAISLVTVSKQVIKGMN
EGSLKWETLSRNPDILNNSIARADGLYNGDCSLAPVLVFLNDHKAETLERVTAVAKAFAD
SHDKEGLQFLLAAGNAGIEAATNEVIKSAELTILILVYICVAVMCLITFRSFAATLCIVL
PLVLTSVLGNALMAYMGIGVKVATLPVVALGVGIGVDYGIYIYSRLESFLRAGLPLQEAY
YQTLRSTGKAVLFTGLCLAIGVCTWIFSAIKFQADMGLMLTFMLLWNMFGALWLLPALAR
FLIKPEKMVGKEGGSIFAH