Protein Info for PP_2001 in Pseudomonas putida KT2440

Annotation: O-succinylhomoserine sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01053: Cys_Met_Meta_PP
  • amino acids 20 to 400 (381 residues), 470.5 bits, E=6.3e-145
• TIGR01325: O-succinylhomoserine sulfhydrylase
  • amino acids 20 to 400 (381 residues), 629.6 bits, E=7.1e-194
• PF00155: Aminotran_1_2
  • amino acids 42 to 203 (162 residues), 56.2 bits, E=7.2e-19
• PF01041: DegT_DnrJ_EryC1
  • amino acids 72 to 208 (137 residues), 28.4 bits, E=2.1e-10
• PF00266: Aminotran_5
  • amino acids 85 to 237 (153 residues), 42.4 bits, E=9.3e-15

Best Hits

Swiss-Prot: 82% identical to METZ_PSEAE: O-succinylhomoserine sulfhydrylase (metZ) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K10764, O-succinylhomoserine sulfhydrylase [EC: 2.5.1.-] (inferred from 100% identity to ppf:Pput_3760)

MetaCyc: 82% identical to O-succinyl-L-homoserine sulfhydrylase (Pseudomonas aeruginosa PAO1)
Cystathionine gamma-synthase. [EC: 2.5.1.48]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carotenoid biosynthesis - General
• Cysteine metabolism
• Methionine metabolism
• Porphyrin and chlorophyll metabolism
• Riboflavin metabolism
• Selenoamino acid metabolism
• Sulfur metabolism
• Terpenoid biosynthesis
• Tryptophan metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-, 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.- or 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See Q88LD4 at UniProt or InterPro

Protein Sequence (403 amino acids)

>PP_2001 O-succinylhomoserine sulfhydrylase (Pseudomonas putida KT2440)
MTDQWDAGRLDSDLEGVGFDTLAVRAGQNRTPEGEHSEALFLTSSYVFRTAADAAARFAG
ETPGNVYSRYTNPSVRAFEERLAAMEGAEQAVGTSTGMAAILAVVMSLCSAGDHVLVSQS
VFGSTISLFEKYFKRFGVEVDYVPLVDLTGWEKAIKANTKLLIVESPSNPLAELVDITAL
SEIAHAQGAMLVVDNCFSTPALQQPLKLGADIVFHSATKFIDGQGRCMGGVVAGRTEQMK
EVVGFLRTAGPTLSPFNAWIFTKGLETLRLRMRAHCESAQALAEWLEQQDGVEKVHYAGL
PSHPQHELAKRQMSGFGAVVSFEVKGGKEGAWRFIDATRVISITTNLGDSKTTIAHPATT
SHGRLSPQEREAAGIRDSLIRVAVGLEDVADLQADLARGLAAL