Protein Info for PP_1982 in Pseudomonas putida KT2440

Annotation: small heat shock protein IbpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 149 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF00011: HSP20" amino acids 45 to 142 (98 residues), 62 bits, see alignment E=2.5e-21

Best Hits

Swiss-Prot: 80% identical to IBPB_AZOVI: 16 kDa heat shock protein B (ibpB) from Azotobacter vinelandii

KEGG orthology group: K04080, molecular chaperone IbpA (inferred from 100% identity to ppu:PP_1982)

Predicted SEED Role

"16 kDa heat shock protein A"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LF1 at UniProt or InterPro

Protein Sequence (149 amino acids)

>PP_1982 small heat shock protein IbpA (Pseudomonas putida KT2440)
MTMTTAFSLAPLFRHSVGFDRFNDLFESAARNEAGSSYPPYNVEKHGDDHYRIVVAAAGF
QEEDLDLQVEKGVLTVTGGKRDNSAAEVTYLHQGIAQRAFKLSFRLADHIEVKAAGLANG
LLSIDLLRIVPEEAKAKRIPINGEKPALN