Protein Info for PP_1904 in Pseudomonas putida KT2440

Annotation: UDP-N-acetylenolpyruvoylglucosamine reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00179: UDP-N-acetylenolpyruvoylglucosamine reductase
  • amino acids 23 to 347 (325 residues), 231.2 bits, E=8e-73
• PF01565: FAD_binding_4
  • amino acids 38 to 168 (131 residues), 91.5 bits, E=3.9e-30
• PF02873: MurB_C
  • amino acids 228 to 350 (123 residues), 99.5 bits, E=1.1e-32

Best Hits

Swiss-Prot: 100% identical to MURB_PSEPK: UDP-N-acetylenolpyruvoylglucosamine reductase (murB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00075, UDP-N-acetylmuramate dehydrogenase [EC: 1.1.1.158] (inferred from 100% identity to ppu:PP_1904)

Predicted SEED Role

"UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158)" in subsystem Peptidoglycan Biosynthesis or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 1.1.1.158)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan recycling I (10/14 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.158

Sequence Analysis Tools

See Q88LM5 at UniProt or InterPro

Protein Sequence (351 amino acids)

>PP_1904 UDP-N-acetylenolpyruvoylglucosamine reductase (Pseudomonas putida KT2440)
MPGAGTGNQGASMTVQWQEQVSLKPYNTFGIDVKARYFSQVQDDQQVRQALGQAQQRGLP
VLVIGGGSNLLLTRDIDALVLHMASRGRRVLSDDGERIVVEAEAGEPWHAFVQWTLAQGY
CGLENLSLIPGTVGAAPMQNVGAYGVEIKDVFAGLTALDRETGELRDFSLAECAFGYRDS
LFKRNPGRWLILRVRFALTRTLHAHLDYGPVRQRLSERGVTELTAQAISDAICSIRREKL
PDPAELGNAGSFFKNPVVTADLVERIRAQYPGVVAYPQADGQVKLAAGWLIEQAGWKGHR
EGDAGVHRLQSLVLVNYGQASGAQMHALARKIQADIFERFGVELEMEPNLY