Protein Info for PP_1829 in Pseudomonas putida KT2440

Annotation: Hydrolase, alpha/beta fold family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF12146: Hydrolase_4" amino acids 77 to 280 (204 residues), 48.4 bits, see alignment E=1.2e-16 PF12697: Abhydrolase_6" amino acids 79 to 285 (207 residues), 49.7 bits, see alignment E=1.2e-16

Best Hits

KEGG orthology group: K06889, (no description) (inferred from 100% identity to ppf:Pput_3881)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LU8 at UniProt or InterPro

Protein Sequence (319 amino acids)

>PP_1829 Hydrolase, alpha/beta fold family (Pseudomonas putida KT2440)
MSRLVALLFLLCTGLAQAAAPTVLQRPIDLDTGQGVLHGSLLLPQQATPPPVVLIIAGSG
PTDRDGNNPASGRVDNLKRLALLLANEHIASVRYDKRGVAASQPATPDERDLSVERYVAD
VVAWSRKLKADPRFGPLILIGHSEGALIASLAAEQAGASAVITLAGSGRPLAEVLHEQLA
QRLPPAQLAGGSALLDRLQAGQTSLDVPAPLRQVFRPSVQPYLISLFQQNPAAAFARLPM
PALIVQGRNDVQVDVADAQRLKAAKPDAQLVLIDGMNHMLRISPKGMSQQRDSYLNPELP
LARELGERIVTFIQHLPSA