Protein Info for PP_1807 in Pseudomonas putida KT2440

Annotation: 2-dehydro-3-deoxyphosphooctonate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 280 PF00793: DAHP_synth_1" amino acids 5 to 272 (268 residues), 233.6 bits, see alignment E=1e-73 TIGR01362: 3-deoxy-8-phosphooctulonate synthase" amino acids 15 to 272 (258 residues), 380.2 bits, see alignment E=2e-118

Best Hits

Swiss-Prot: 100% identical to KDSA2_PSEPK: 2-dehydro-3-deoxyphosphooctonate aldolase 2 (kdsA2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01627, 2-dehydro-3-deoxyphosphooctonate aldolase (KDO 8-P synthase) [EC: 2.5.1.55] (inferred from 100% identity to ppu:PP_1807)

MetaCyc: 74% identical to 3-deoxy-D-manno-octulosonate 8-phosphate synthase (Escherichia coli K-12 substr. MG1655)
3-deoxy-8-phosphooctulonate synthase. [EC: 2.5.1.55]

Predicted SEED Role

"2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)" in subsystem KDO2-Lipid A biosynthesis (EC 2.5.1.55)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.55

Use Curated BLAST to search for 2.5.1.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LX0 at UniProt or InterPro

Protein Sequence (280 amino acids)

>PP_1807 2-dehydro-3-deoxyphosphooctonate aldolase (Pseudomonas putida KT2440)
MIKINSTIECSNSAPFVLFGGINVLESEDLALTACAEYVRVTQKLGIPYVFKASFDKANR
SSIHSYRGPGMEEGLRIFEKVKAEFGVPIITDVHEIYQTAPVAEVVDVLQLPAFLARQTD
LVVALAKTGKPVNIKKPQFLSPSQMQNIVHKFKEAGNDQLILCDRGTCMGYDNLIVDMLG
FGVMKRTCQDLPIIFDVTHALQNRDPSGAASGGRREQVVELARAGMGVGLAGLFLEAHPN
PDQAKCDGPSALPLDKLEPFLAQIKALDDLVKSFPQLTIA