Protein Info for PP_1770 in Pseudomonas putida KT2440

Annotation: 3-phosphoshikimate 1-carboxyvinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03807: F420_oxidored
  • amino acids 16 to 95 (80 residues), 24.4 bits, E=7.5e-09
• PF02153: PDH_N
  • amino acids 28 to 182 (155 residues), 124.5 bits, E=5.6e-40
• PF20463: PDH_C
  • amino acids 188 to 284 (97 residues), 98.4 bits, E=5.4e-32
• PF00275: EPSP_synthase
  • amino acids 317 to 730 (414 residues), 386.7 bits, E=2.4e-119
• TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase
  • amino acids 324 to 737 (414 residues), 419.5 bits, E=6.9e-130

Best Hits

KEGG orthology group: K00210, prephenate dehydrogenase [EC: 1.3.1.12] K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 100% identity to ppu:PP_1770)

Predicted SEED Role

"Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43, EC 2.5.1.19)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• L-tyrosine biosynthesis I (3/3 steps found)
• L-tyrosine biosynthesis III (3/4 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• 4-hydroxy-3-prenylbenzoate biosynthesis (1/4 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Novobiocin biosynthesis
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.12 or 1.3.1.43 or 2.5.1.19

Sequence Analysis Tools

See Q88M05 at UniProt or InterPro

Protein Sequence (746 amino acids)

>PP_1770 3-phosphoshikimate 1-carboxyvinyltransferase (Pseudomonas putida KT2440)
MVNAAKTKPAPIIDRLVVVGLGLIGGSFAKGLRESGLCREVVGVDLDAPSRKQAVALGVV
DRCEEDLAAACVGADVIQLAVPILAMEKVLARLALLDLGDAIITDVGSAKGNVVREARAV
FGARLPRFVPGHPIAGSEQSGVEASNATLFRRHKVILTPLAETDPAALVLVDRLWRALDA
DVEHMSVERHDEVLAATSHLPHLLAFGLVDSLAKRNENLEIFRYAAGGFRDFTRIAGSDP
TMWHDIFLANRDAVLRTLDTFRSDLDALRDAIAEGDGHQLLGVFTRARVAREHFSKILAR
RAYVDAMNANDLIFQAQPGGRLNGRIRVPGDKSISHRSIMLGSLAEGTTEVEGFLEGEDA
LATLQAFRDMGVVIEGPNHGRVTIHGVGLHGLKPPPGPLYVGNSGTSMRLLSGLLAGQPF
DVTMTGDASLSKRPMNRVANPLREMGAVVETGPDGRPPLTIRGGHKLKGLTYTLPMASAQ
VKSCLLLAGLYAEGKTTVTEPAPTRDHTERMLRGFGYSVDSHGPVASLQSGGKLTATRIE
VPADISSAAFFLVAASIAEGSELVLEHVGINPTRTGVIDILRLMGGDITLENQREVGGEP
VADLRVRGAKLKGIDIPEALVPLAIDEFPVLFVAAACAEGRTVLRGAEELRVKESDRIQV
MADGLTTLGIKCEPTPDGIIIDGGQMGGGEVHGHGDHRIAMAFSVASLRASAPIRIHDCA
NVATSFPNFLALCAEVGIRVAEEGKS