Protein Info for PP_1764 in Pseudomonas putida KT2440

Annotation: Phosphoglycolate phosphatase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 PF00702: Hydrolase" amino acids 4 to 180 (177 residues), 100.6 bits, see alignment E=3.1e-32 PF13419: HAD_2" amino acids 6 to 186 (181 residues), 117.6 bits, see alignment E=1.4e-37 PF12710: HAD" amino acids 6 to 174 (169 residues), 42.6 bits, see alignment E=2e-14 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 6 to 212 (207 residues), 172 bits, see alignment E=2e-54 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 80 to 180 (101 residues), 40.9 bits, see alignment E=4e-14 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 124 to 183 (60 residues), 30.7 bits, see alignment E=4.8e-11 PF13242: Hydrolase_like" amino acids 142 to 208 (67 residues), 42.1 bits, see alignment E=1.3e-14

Best Hits

Swiss-Prot: 100% identical to MUPP_PSEPK: N-acetylmuramic acid 6-phosphate phosphatase (mupP) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 100% identity to ppf:Pput_3950)

MetaCyc: 100% identical to N-acetyl-beta-muramate 6-phosphate phosphatase (Pseudomonas putida KT2440)
RXN-18659 [EC: 3.1.3.105]

Predicted SEED Role

"Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.105 or 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88M11 at UniProt or InterPro

Protein Sequence (223 amino acids)

>PP_1764 Phosphoglycolate phosphatase 2 (Pseudomonas putida KT2440)
MRLRAVLFDMDGTLLDTAPDFIAICQAMLAERGLPAVDDNLIRGVISGGARAMVATAFAM
DPEADGFEALRLEFLERYQRDCAVHSKLFEGMAELLADIEKGNLLWGVVTNKPVRFAEPI
MQQLGLAERSALLICPDHVKNSKPDPEPLILACKTLNLDPASVLFVGDDLRDIESGRDAG
TRTAAVRYGYIHPEDNPNNWGADVVVDHPLELRKVIDSALCGC