Protein Info for PP_1743 in Pseudomonas putida KT2440

Annotation: acetate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 554 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 38 to 57 (20 residues), see Phobius details amino acids 79 to 101 (23 residues), see Phobius details amino acids 107 to 126 (20 residues), see Phobius details amino acids 152 to 173 (22 residues), see Phobius details amino acids 185 to 205 (21 residues), see Phobius details amino acids 213 to 233 (21 residues), see Phobius details amino acids 266 to 290 (25 residues), see Phobius details amino acids 302 to 327 (26 residues), see Phobius details amino acids 360 to 387 (28 residues), see Phobius details amino acids 408 to 427 (20 residues), see Phobius details amino acids 433 to 457 (25 residues), see Phobius details amino acids 468 to 491 (24 residues), see Phobius details amino acids 503 to 522 (20 residues), see Phobius details TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 67 to 471 (405 residues), 212.5 bits, see alignment E=5.7e-67 PF00474: SSF" amino acids 67 to 473 (407 residues), 440.8 bits, see alignment E=2.4e-136

Best Hits

Swiss-Prot: 84% identical to ACTP_YERE8: Cation/acetate symporter ActP (actP) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K14393, cation/acetate symporter (inferred from 100% identity to ppu:PP_1743)

MetaCyc: 81% identical to acetate/glycolate:cation symporter (Escherichia coli K-12 substr. MG1655)
RXN0-1981; RXN0-5111; TRANS-RXN0-576

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88M32 at UniProt or InterPro

Protein Sequence (554 amino acids)

>PP_1743 acetate permease (Pseudomonas putida KT2440)
MIRQVKALAVLACGAFAPAVWAADALTGEVQKQPLNVSAIAMFVAFVAFTLGITYWASKR
NKSASDYYAAGGKITGFQNGLAIAGDYMSAASFLGISALVFTSGYDGLIYSIGFLVGWPI
ILFLIAERLRNLGKYTFADVASYRLGQKEIRTLSASGSLVVVAFYLIAQMVGAGKLIELL
FGLDYHVAVILVGILMCLYVLFGGMLATTWVQIIKAVLLLSGASFMALMVMKHVGFDFNT
LFSEAIKVHAKGEAIMSPGGLVKDPISAFSLGLALMFGTAGLPHILMRFFTVSDAKEARK
SVLYATGFIGYFYILTFIIGFGAILLVSTNPEFKDAAGALLGGNNMAAVHLANAVGGSVF
LGFISAVAFATILAVVAGLTLAGASAVSHDLYASVWRKGKANDKDEIRVSKITTVALGVL
AIGLGILFEKQNIAFMVGLAFSIAASCNFPVLLLSMYWKKLTTRGAMIGGWLGLVSAVTL
MILGPTIWVQILGHEKPIYPYEYPALFSMAIAFVSIWFFSVTDKSKAAEDERALFFPQFV
RSQTGLGASGAVSH