Protein Info for PP_1617 in Pseudomonas putida KT2440

Annotation: S-formylglutathione hydrolase / S-lactoylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 6 to 281 (276 residues), 421.2 bits, E=1e-130
• PF00756: Esterase
  • amino acids 23 to 275 (253 residues), 225 bits, E=6.3e-71

Best Hits

Swiss-Prot: 63% identical to SFGH2_ECOSM: S-formylglutathione hydrolase YeiG (yeiG) from Escherichia coli (strain SMS-3-5 / SECEC)

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 100% identity to ppu:PP_1617)

MetaCyc: 62% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (5/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Sequence Analysis Tools

See Q88MF4 at UniProt or InterPro

Protein Sequence (284 amino acids)

>PP_1617 S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Pseudomonas putida KT2440)
MSLDNISCQKSFGGWHKRYRHHSKVLGCDMVFAVYLPPQAEQGEKLPVLYWLSGLTCTDE
NFMQKAGAQRLAAELGLIIVAPDTSPRGEQVPGDPDGAWDFGLGAGFYLNATQQPWAQHY
RMHDYVVEELPALIEAHFPASGERSISGHSMGGHGALVCALRNPGRYRSVSAFSPISNPM
DCPWGEKAFSRYLGEDRGRWREWDASVLLAETPAGQCPPLLVDQGDRDDFLEKQLKPEAL
EQAARKGGHAMTLRMQPGYDHSYYFIASFIEEHLRHHAVALGRV