Protein Info for PP_1596 in Pseudomonas putida KT2440

Annotation: CTP:phosphatidate cytidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 271 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 28 to 44 (17 residues), see Phobius details amino acids 56 to 72 (17 residues), see Phobius details amino acids 78 to 96 (19 residues), see Phobius details amino acids 105 to 125 (21 residues), see Phobius details amino acids 134 to 155 (22 residues), see Phobius details amino acids 176 to 193 (18 residues), see Phobius details amino acids 199 to 221 (23 residues), see Phobius details amino acids 249 to 267 (19 residues), see Phobius details PF01148: CTP_transf_1" amino acids 3 to 264 (262 residues), 215.7 bits, see alignment E=5.6e-68

Best Hits

Swiss-Prot: 74% identical to CDSA_PSEAE: Phosphatidate cytidylyltransferase (cdsA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00981, phosphatidate cytidylyltransferase [EC: 2.7.7.41] (inferred from 100% identity to ppf:Pput_4181)

Predicted SEED Role

"Phosphatidate cytidylyltransferase (EC 2.7.7.41)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.7.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MH5 at UniProt or InterPro

Protein Sequence (271 amino acids)

>PP_1596 CTP:phosphatidate cytidyltransferase (Pseudomonas putida KT2440)
MLKQRIITALILLPVALGGFFLLNGGDFALFIGFVVTLGAWEWARLAGLMAQPLRIAYAA
VVAGALMLLHILPELAPWVLGAAVIWWGLATWLVLTYPRSSDLWASAACRLLIGLLVLLP
AWQGLVLLKHWPLGNWLILSVMVLVWAADIGAYFSGRAFGKRKLAPQVSPGKSWEGVYGG
LAVSLLITLGVGISRDWGFGQILLGLLGAALLVMSSVVGDLTESMFKRRSGIKDSSNLLP
GHGGVLDRIDSLTAAIPIFAVLLWAAEWGVM