Protein Info for PP_1590 in Pseudomonas putida KT2440

Annotation: methionine aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00500: methionine aminopeptidase, type I
  • amino acids 4 to 249 (246 residues), 341.8 bits, E=1.2e-106
• PF00557: Peptidase_M24
  • amino acids 12 to 241 (230 residues), 196.2 bits, E=3e-62

Best Hits

Swiss-Prot: 67% identical to MAP1_ECO57: Methionine aminopeptidase (map) from Escherichia coli O157:H7

KEGG orthology group: K01265, methionyl aminopeptidase [EC: 3.4.11.18] (inferred from 99% identity to ppg:PputGB1_1145)

MetaCyc: 67% identical to methionine aminopeptidase (Escherichia coli K-12 substr. MG1655)
Methionyl aminopeptidase. [EC: 3.4.11.18]

Predicted SEED Role

"Methionine aminopeptidase (EC 3.4.11.18)" (EC 3.4.11.18)

MetaCyc Pathways

• Arg/N-end rule pathway (eukaryotic) (8/14 steps found)
• Ac/N-end rule pathway (6/21 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.18

Use Curated BLAST to search for 3.4.11.18

Sequence Analysis Tools

See Q88MI1 at UniProt or InterPro

Protein Sequence (260 amino acids)

>PP_1590 methionine aminopeptidase (Pseudomonas putida KT2440)
MTVTIKTAEDIEKMRIAGRLAAEVLEMIEEHVKPGVTTEELDRLCHDYIVNVQQAIPAPL
NYKGYPKSICTSINHVVCHGIPNDKPLKDGDTLNIDVTVIKDGYHGDTSRMFHVGNVPVW
AERLSKVTQECMYKAIELVKPGCRLGDIGEVIQKHAEKNGFSVVREFCGHGIGKVFHEEP
QILHYGRAGTGMELKEGMTFTIEPMINQGKADTKVLGDGWTAITKDRKLSAQWEHTLVVT
ATGYEIFTLRKDDTIPRTSA