Protein Info for PP_1587 in Pseudomonas putida KT2440

Annotation: NhaP-type Na+/H+ and K+/H+ antiporters

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details amino acids 39 to 40 (2 residues), see Phobius details transmembrane" amino acids 21 to 38 (18 residues), see Phobius details amino acids 59 to 87 (29 residues), see Phobius details amino acids 101 to 122 (22 residues), see Phobius details amino acids 130 to 151 (22 residues), see Phobius details amino acids 172 to 192 (21 residues), see Phobius details amino acids 198 to 218 (21 residues), see Phobius details amino acids 232 to 249 (18 residues), see Phobius details amino acids 255 to 274 (20 residues), see Phobius details amino acids 286 to 305 (20 residues), see Phobius details amino acids 325 to 346 (22 residues), see Phobius details amino acids 375 to 399 (25 residues), see Phobius details amino acids 411 to 434 (24 residues), see Phobius details TIGR00831: Na+/H+ antiporter" amino acids 26 to 562 (537 residues), 431.6 bits, see alignment E=2e-133 PF00999: Na_H_Exchanger" amino acids 31 to 435 (405 residues), 223.6 bits, see alignment E=1.9e-70

Best Hits

KEGG orthology group: K03316, monovalent cation:H+ antiporter, CPA1 family (inferred from 100% identity to ppu:PP_1587)

Predicted SEED Role

"Na+/H+ antiporter" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MI4 at UniProt or InterPro

Protein Sequence (566 amino acids)

>PP_1587 NhaP-type Na+/H+ and K+/H+ antiporters (Pseudomonas putida KT2440)
MAMFRLAIVSGSQRKLSRMQSAYTVLILLTLVSLSKLVGRVIPLPLPLVQIAAGALLAWP
TLGLHVALDPELFLFLFLPPLLFADGWRIPKRELWRIRGPVVALAVGLVLFTVVGAGYFI
HWLLPSIPLPVAFALAAVLSPTDAVAVSAIAQDRLPTPLMHMLQGEALMNDASGLVTFKF
ALAAAITGVFSLADASFSFVLVALGGLAVGVVLSWLIGRLRMWMITRGWDDPATHVVFML
LLPFAAYVLAERLGVSGILSAVAAGMMQSWLDLLPRQTSTRLLNRSVWSLLEFAFNGLIF
LLLGLQLPDIIKAVVSHEATLWPTLAYRCLDVVAIFAALIMLRFIWVQSIWRSIGVVRRW
RGKPALVLMPTARSCWLLTLGGVRGAVTLAGVMSIPLLIGAGTAFPERDLLIFIAAGVIL
LSLISACIALPILLRGVTKSPDERLHQEVQEAWRRTAEAAIHALEAEEVIDSNAPQDAAQ
ATLATELKARLMAEYRDELESYDDSAEAKALAAQMDLLERRLRLRALRAQRLELYNLHRQ
HLVGDEVVRQVLGELDMSEANLGSVK