Protein Info for PP_1490 in Pseudomonas putida KT2440

Annotation: Probable biofilm formation methyltransferase WspC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 PF03705: CheR_N" amino acids 4 to 51 (48 residues), 27 bits, see alignment 1e-09 PF01739: CheR" amino acids 67 to 240 (174 residues), 124.5 bits, see alignment E=1.3e-39 PF08241: Methyltransf_11" amino acids 178 to 236 (59 residues), 21.5 bits, see alignment E=1.1e-07 PF13181: TPR_8" amino acids 355 to 388 (34 residues), 23.1 bits, see alignment 2e-08 PF14559: TPR_19" amino acids 368 to 421 (54 residues), 30.5 bits, see alignment 1.3e-10

Best Hits

Swiss-Prot: 100% identical to WSPC_PSEPK: Probable biofilm formation methyltransferase WspC (wspC) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K13486, chemotaxis protein methyltransferase WspC (inferred from 100% identity to ppu:PP_1490)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MS8 at UniProt or InterPro

Protein Sequence (424 amino acids)

>PP_1490 Probable biofilm formation methyltransferase WspC (Pseudomonas putida KT2440)
MNEQRFFRFLRERIGLDVESVGAPMVERALRQRCVAAGAMDLDDYWLRLQQSADEQQALI
EAVIVPETWFFRYPESFTALASLAHKRLAQLAGARPLRLLSLPCSTGEEPYSLAMALFDA
GMAPGAFLVDGMDISPSSVAKAGQAVYGRNAFRGSELGFRERYFDALDEGHRLHERVRQQ
VSLRVGNVLDPALASRDGLYDFVFCRNLLIYFDVPTQQRVFEVLKRLLHPQGVLFIGPAE
GSLLARMGMRPLGIAQSFAYVRHEGDSAPLAAAPAQTAKRAFTTLPAPVYPQPSVPLPRS
RRVLPVAARPARAREHSHEGASELLAGIARLANAGASEQARSECQRYLSQYPPSAQVYYW
LGLLSDTEGDAQQALSHYRKALYLEPQHPEALVHLAALLAAQGDLAGARRLQERAARAGR
ESER