Protein Info for PP_1446 in Pseudomonas putida KT2440

Annotation: TonB-dependent receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF07715: Plug" amino acids 40 to 144 (105 residues), 73.9 bits, see alignment E=1.5e-24 PF00593: TonB_dep_Rec_b-barrel" amino acids 208 to 649 (442 residues), 172.6 bits, see alignment E=2.8e-54

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 100% identity to ppu:PP_1446)

Predicted SEED Role

"Zinc-regulated outer membrane receptor" in subsystem Transport of Zinc

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MX2 at UniProt or InterPro

Protein Sequence (680 amino acids)

>PP_1446 TonB-dependent receptor (Pseudomonas putida KT2440)
MLYTPLRLSPLAVALWLAASPSQAVELEPQVITANPLGNRQLAAPSTVLEGDNLLQQQHG
SLGETLNKQPGVASTWFGPGASRPVIRGLDGDRIRILRNGVGALDASSLSYDHAVPLDPV
TVERVEIVRGPAALLYGGNAIGGVVNTFDNRIPDSPIEGIHGAGELRYGGADTTRSSAGK
LEAGDGAFALHLDANSRQFNDLRIPGYARSAKVRDADEPGSKHRLENSDGRQDGGAIGGA
YHWDHGYAGLSYSRYDSNYGSVAESGVRLDMKQDHYAFASELRDLDGPFSSVKVDAGYTD
YEHSEIEEGEVHTTFKNKGYEARIEARHQPLGPIEGVIGAQVSRNEFSALGEEAFVPHTD
TDSLALFMLEQWQATERLNLSLGARMEHTRVDPDAKGNENFVDADSASSFNAFSLSSGAV
YQLDPIWSLAANLGYTERAPTFYELYANGAHVATGAFEVGDASLNKEKAISADLALRFDN
GTHKGSVGLFYSHFRNYIGLIGTGNTREGEHDHEEEDHDHDHDHEGFPEYQYQGVRARFY
GIEAQDRWQLAENRYGSFALELSGDYTRAKNLDSGEPLPRIAPLRLNSGLVWELDRWQAR
VDVQHAASQHRKPANETSTDGYTTLGASVGYRFDIGQSQWLAFVRGENLTDQTVRYASSI
LRDIAPAPGRSVEVGLRTTF