Protein Info for PP_1428 in Pseudomonas putida KT2440

Annotation: Sigma factor AlgU negative regulatory protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 196 transmembrane" amino acids 85 to 105 (21 residues), see Phobius details PF03872: RseA_N" amino acids 7 to 76 (70 residues), 50.7 bits, see alignment E=2.3e-17 PF03873: RseA_C" amino acids 118 to 178 (61 residues), 38.6 bits, see alignment E=1e-13

Best Hits

KEGG orthology group: K03597, sigma-E factor negative regulatory protein RseA (inferred from 100% identity to ppf:Pput_4293)

Predicted SEED Role

"Sigma factor RpoE negative regulatory protein RseA" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MZ0 at UniProt or InterPro

Protein Sequence (196 amino acids)

>PP_1428 Sigma factor AlgU negative regulatory protein (Pseudomonas putida KT2440)
MSREALQESLSAVMDNEADELELRRVLNAVDDAETRATWSRYQVARAAMHKELLLPNLDI
ASAVSAALADEAVPAKVKKGPWRSIGRLAVAASVTVAVLAGVRMYNQDEITGAELAAQQP
VQQGLSVPQAQGPAVLAGYSESSEQPTGPMANGVLQNQAGWDQRLPGYLRQHAQESALKG
TETALPYARAASLENR