Protein Info for PP_1402 in Pseudomonas putida KT2440

Annotation: C4-dicarboxylate transport sensor protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 301 to 320 (20 residues), see Phobius details PF00512: HisKA" amino acids 363 to 427 (65 residues), 35.5 bits, see alignment E=8.7e-13 PF02518: HATPase_c" amino acids 472 to 579 (108 residues), 73.7 bits, see alignment E=1.7e-24

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 100% identity to ppu:PP_1402)

Predicted SEED Role

"C4-dicarboxylate transport sensor protein"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N14 at UniProt or InterPro

Protein Sequence (585 amino acids)

>PP_1402 C4-dicarboxylate transport sensor protein (Pseudomonas putida KT2440)
MPFSFRALRLGLITLLIVLGTALSAGWAMHQAKRQAMEDDAKRASQQLGLYANALHTLID
RYRALPAVLALDPELIAALRGPVDEKVQNALNLKLERINGAANSSTLELLDRTGLAIAAS
NWRLPSSYVGSNYGFRPYFKQTRSQGSGRFYAVGVTSGVPGYFLASAVNDEHGRFLGAMV
VKLEFPELEREWRQGNDILLVSDARGITFIANQDGWRYRELQPLSGADRADLAETRQYDK
QPLVPLHHQVLTRFAPYSTLSRVQGPEGSTEYLWESLPLEGENWTLHLLRKPQVAADGRN
AALGAAAVWLSLVFAALFVSQRLRLARLRQRSRQELERQVEERTRELRTAQEGLVQSAKL
AALGQMSAAMAHEINQPLTTQRMQLETLRLLLDHGRHDEARQALEPLEQMLTRMAALTSH
LKTFARNSPMGLRERLDLATVVDQALHLLEARLRNEEVEVALYLARPAWVRGDAIRLEQV
LINLLHNALDAMLDKRYKRLEIRIEPDEDLWRLSVLDSGGGIAEADLAKVFDPFFTTKPV
GEGLGLGLAISYGIVHEAGGQLRAENLPGGARLSLTLPRDLEPVC