Protein Info for PP_1400 in Pseudomonas putida KT2440

Annotation: Alpha-ketoglutarate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 transmembrane" amino acids 41 to 59 (19 residues), see Phobius details amino acids 66 to 90 (25 residues), see Phobius details amino acids 98 to 117 (20 residues), see Phobius details amino acids 123 to 144 (22 residues), see Phobius details amino acids 165 to 187 (23 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 249 to 267 (19 residues), see Phobius details amino acids 286 to 303 (18 residues), see Phobius details amino acids 315 to 334 (20 residues), see Phobius details amino acids 340 to 364 (25 residues), see Phobius details amino acids 376 to 397 (22 residues), see Phobius details amino acids 409 to 427 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 32 to 240 (209 residues), 105 bits, see alignment E=4.5e-34 amino acids 225 to 433 (209 residues), 38.2 bits, see alignment E=8.4e-14 PF07690: MFS_1" amino acids 33 to 380 (348 residues), 102.7 bits, see alignment E=2e-33

Best Hits

Swiss-Prot: 56% identical to KGTP_ECOLI: Alpha-ketoglutarate permease (kgtP) from Escherichia coli (strain K12)

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 99% identity to ppw:PputW619_1041)

MetaCyc: 56% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"Dicarboxylate MFS transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N16 at UniProt or InterPro

Protein Sequence (439 amino acids)

>PP_1400 Alpha-ketoglutarate permease (Pseudomonas putida KT2440)
MDNATSLPAGAATAPAAEKTTASRLKSIFSGSIGNMVEWYDWYVYAAFSLYFAKAFFPAG
DSTAQLLNTAAIFAVGFLMRPIGGWLMGLYADRKGRKAALMASVLLMCAGSLVIALTPGY
ETIGVAAPVLLVIARLLQGLSVGGEYGTSATYLSEMASKERRGFFSSFQYVTLISGQLIA
LAVLIVLQQTLTTEQLYAWGWRVPFVIGALCAVVALYLRRGMEETASFTKKEKAKESLMR
TLLRHPKELMTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSISDSTTISAATLFLFMCLQ
PMIGGLSDKIGRRPILIAFGVLGTLFTVPILSTLHTIQTWWGAFFLIMAALIIVSGYTSI
NAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYVALWFKSAGMESGFYWYVTACIACS
LLVYATMKDTKQHSRITTE