Protein Info for PP_1379 in Pseudomonas putida KT2440

Annotation: 3-carboxy-cis,cis-muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02426: 3-carboxy-cis,cis-muconate cycloisomerase
  • amino acids 5 to 348 (344 residues), 493.2 bits, E=1.7e-152
• PF00206: Lyase_1
  • amino acids 11 to 300 (290 residues), 276.7 bits, E=2.9e-86
• PF10397: ADSL_C
  • amino acids 364 to 440 (77 residues), 64.6 bits, E=8.6e-22

Best Hits

Swiss-Prot: 95% identical to PCAB_PSEPU: 3-carboxy-cis,cis-muconate cycloisomerase (Fragment) (pcaB) from Pseudomonas putida

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 100% identity to ppf:Pput_4344)

MetaCyc: 95% identical to subunit of 3-carboxymuconate cycloisomerase (Pseudomonas putida)
3-carboxy-cis,cis-muconate cycloisomerase. [EC: 5.5.1.2]

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (19/35 steps found)
• superpathway of aerobic toluene degradation (13/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.5.1.2

Sequence Analysis Tools

See Q88N37 at UniProt or InterPro

Protein Sequence (450 amino acids)

>PP_1379 3-carboxy-cis,cis-muconate cycloisomerase (Pseudomonas putida KT2440)
MSNQLFDAYFTAPAMREIFSDRGRLQGMLDFEAALARAEASAGLVPHSAVAAIEAACQAE
RYDVGALANAIATAGNSAIPLVKALGKVIATGVPEAERYVHLGATSQDAMDTGLVLQLRD
ALDLIEADLGKLADTLSQQALKHADTPLVGRTWLQHATPVTLGMKLAGVLGALTRHRQRL
QELRPRLLVLQFGGASGSLAALGSKAMPVAEALAEQLKLTLPEQPWHTQRDRLVEFASVL
GLVAGSLGKFGRDISLLMQTEAGEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAATRVPG
LLSTLFAAMPQEHERSLGLWHAEWETLPDICCLVSGALRQAQVIAEGMEVDAARMRRNLD
LTQGLVLAEAVSIVLAQRLGRDRAHHLLEQCCQRAVAEQRHLRAVLGDEPQVSAELSGEE
LDRLLDPAHYLGQARVWVARAVSEHQRFTA