Protein Info for PP_1361 in Pseudomonas putida KT2440

Annotation: 60 kDa chaperonin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 TIGR02348: chaperonin GroL" amino acids 3 to 527 (525 residues), 843.6 bits, see alignment E=2.8e-258 PF00118: Cpn60_TCP1" amino acids 23 to 522 (500 residues), 240.9 bits, see alignment E=1.3e-75

Best Hits

Swiss-Prot: 100% identical to CH60_PSEPK: 60 kDa chaperonin (groL) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K04077, chaperonin GroEL (inferred from 100% identity to ppf:Pput_4363)

MetaCyc: 80% identical to chaperonin GroEL (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Heat shock protein 60 family chaperone GroEL" in subsystem GroEL GroES or Staphylococcal pathogenicity islands SaPI

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N55 at UniProt or InterPro

Protein Sequence (546 amino acids)

>PP_1361 60 kDa chaperonin (Pseudomonas putida KT2440)
MAAKDVKFGDSARKKMLVGVNVLADAVKATLGPKGRNVVLAKSFGAPTITKDGVSVAKEI
ELKDAFENMGAQLVKEVASKANDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKRGI
DKATAAVVAELKNLSKPCADSKAIAQVGTISANSDNSIGEIIAEAMEKVGKEGVITVEEG
SGLENELSVVEGMQFDRGYLSPYFVNKPDTMVAELEGPLLLLVDKKISNIRELLPVLEAV
AKAGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAVLTGGQV
ISEEIGLSLETATLEHLGNAKRVILSKENTTIIDGAGADTEIEARVKQIRAQIEETSSDY
DREKLQERLAKLAGGVAVIKVGAGTEVEMKEKKARVEDALHATRAAVEEGVVPGGGVALV
RALAAIIDLKGDNEDQNVGIALLRRAVESPLRQITANAGDEPSVVADKVKQGSGNFGYNA
ATGEYGDMIEMGILDPAKVTRSALQAAASIGGLMITTEAMVADLPEDKPAAGMPDMGGMG
GMGGMM