Protein Info for PP_1350 in Pseudomonas putida KT2440

Annotation: Sensory box histidine kinase/response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 676 transmembrane" amino acids 16 to 42 (27 residues), see Phobius details amino acids 156 to 179 (24 residues), see Phobius details PF00672: HAMP" amino acids 185 to 234 (50 residues), 31.9 bits, see alignment 3.5e-11 TIGR00229: PAS domain S-box protein" amino acids 288 to 403 (116 residues), 30 bits, see alignment E=2.4e-11 PF00989: PAS" amino acids 289 to 394 (106 residues), 39.8 bits, see alignment E=1e-13 PF08448: PAS_4" amino acids 294 to 397 (104 residues), 33.2 bits, see alignment E=1.3e-11 PF00512: HisKA" amino acids 415 to 500 (86 residues), 29.1 bits, see alignment E=2.1e-10 PF02518: HATPase_c" amino acids 553 to 664 (112 residues), 87.9 bits, see alignment E=1.6e-28

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1350)

Predicted SEED Role

"Sensory box histidine kinase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N65 at UniProt or InterPro

Protein Sequence (676 amino acids)

>PP_1350 Sensory box histidine kinase/response regulator (Pseudomonas putida KT2440)
MTLRQRLENLPVGQKLLAALLVLLVTILLMANLTFISAAYWITQESMAPQAMQTIGRLVA
NPQLAARAGDTPETARALLKELESYTPLRAAAVYGGDGRMLAQLQHGEPLALPKRFRNID
GWRLMEFRSTQLIRIPRDANPPAHLLLVASSELPTAFYTGTLSASLGILVFSVLLWIVIA
RQIKRLITQPINQLEELSRQVTREESYALRAQRGNDDEIGSLAEAFNTMLSRIEAREQQL
KSARDEFQSAYDQAQGLAEETRHTNRKLELEVQVRSKIEKKLTGFQNYLNSIIDSMPSAL
IALDEQLYVTQWNHEATVLSGTPLDEALNQPVFIAFEPLKPFLPQLKETVEKHRVAKIER
VTWPKGDDLRHYALTFYPLTGGGGRGVVIRIDDITQRLSLEEMMVQSEKMLSVGGLAAGM
AHEINNPLGAILHNVQNIRRRLSSDLPRNQEQAEELGIDLATINRYLDSREVPQLLDGIQ
QAGARAAKIVTHMLSFSRRSNRQLVPCELPALIDQALEIAGNDFDLAIGFDFKGQAIVRQ
FDPDLGPVPCTANELEQVLLNLLKNAAQAIHQRPQPSEPGRITLRTRLNPPWAEVQVEDN
GVGMPEAVRKRTFEPFFTTKEIGQGTGLGLSVSYFIITNNHKGQMEVQSTPGQGTCFTLR
LPLTQPAAAVPARTEQ