Protein Info for PP_1304 in Pseudomonas putida KT2440

Annotation: sulfate adenylyltransferase subunit 1/adenylyl-sulfate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 23 to 229 (207 residues), 162.2 bits, E=2.2e-51
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 25 to 432 (408 residues), 702.2 bits, E=3.4e-215
• PF01926: MMR_HSR1
  • amino acids 31 to 166 (136 residues), 25.5 bits, E=2.4e-09
• TIGR00231: small GTP-binding protein domain
  • amino acids 31 to 210 (180 residues), 49 bits, E=8.5e-17
• PF03144: GTP_EFTU_D2
  • amino acids 263 to 322 (60 residues), 30.7 bits, 7e-11
• PF01583: APS_kinase
  • amino acids 464 to 615 (152 residues), 167.8 bits, E=3.8e-53

Best Hits

Swiss-Prot: 87% identical to CYSNC_PSEAE: Bifunctional enzyme CysN/CysC (cysNC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 100% identity to ppu:PP_1304)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• sulfite oxidation III (2/3 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (2/5 steps found)
• selenate reduction (2/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (2/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (2/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (6/12 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Sequence Analysis Tools

See Q88NA8 at UniProt or InterPro

Protein Sequence (633 amino acids)

>PP_1304 sulfate adenylyltransferase subunit 1/adenylyl-sulfate kinase (Pseudomonas putida KT2440)
MSHQSDLISEDILAYLAQHERKELLRFLTCGNVDDGKSTLIGRLLHDSKMIYEDHLEAIT
RDSKKVGTTGEEVDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
MATGASTCDLAIILVDARYGVQTQTRRHSYIASLLGIKHIVVAVNKMDLKGFDEGVFESI
KADYLKFAEAINLTPSSLHFVPMSALKGDNVVNHSEQSPWYAGPTLMEILETVEVSADRN
FTDLRFPVQYVNRPNLNFRGFAGTIASGVVHKGDEIVVLPSGKSSRVKSIVTYEGELENA
GPGQAVTLTMEDEIDISRGDLLVHADNVPPVTDQFDAMLVWMAEEPMLPGKKYDIKRATS
YVPGSIASITHKVDVNTLEQGAASALQLNEIGRVKVALDTSIALDGYDSNRTTGAFIVID
RLTNGTVGAGMIIAPPVLPHGSTGHHGKQAHVSTEERALRFGQQPATVLFSGLSGAGKST
LAYAVERKLFDMGRAVYVLDGQNLRHDLNKGLPQDRAGRTENWRRAAHVARQFNEAGMLT
LAAFVAPDAEGREQAKALIGKERLVTVYVQASPLVCRERDPQGLYAAGGDNIPGESFPFD
VPLDADLVIDTQATSVDEGVKQVLDVLRQRGAI