Protein Info for PP_1277 in Pseudomonas putida KT2440

Annotation: mannose-6-phosphate isomerase/mannose-1-phosphate guanylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 2 to 469 (468 residues), 739.9 bits, E=6.1e-227
• PF00483: NTP_transferase
  • amino acids 4 to 286 (283 residues), 222.1 bits, E=1.7e-69
• PF12804: NTP_transf_3
  • amino acids 5 to 130 (126 residues), 31.6 bits, E=3.5e-11
• PF01050: MannoseP_isomer
  • amino acids 315 to 465 (151 residues), 260.2 bits, E=1.1e-81
• PF07883: Cupin_2
  • amino acids 381 to 449 (69 residues), 44.4 bits, E=2.2e-15

Best Hits

Swiss-Prot: 100% identical to ALGA_PSEPK: Alginate biosynthesis protein AlgA (algA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 99% identity to ppg:PputGB1_4572)

MetaCyc: 83% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]

Predicted SEED Role

No annotation

MetaCyc Pathways

• GDP-mannose biosynthesis (4/4 steps found)
• colanic acid building blocks biosynthesis (9/11 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• mannitol degradation II (2/4 steps found)
• mannitol biosynthesis (1/3 steps found)
• 1,5-anhydrofructose degradation (2/5 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (7/14 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.13, 2.7.7.22, 5.3.1.8

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

See Q88ND5 at UniProt or InterPro

Protein Sequence (485 amino acids)

>PP_1277 mannose-6-phosphate isomerase/mannose-1-phosphate guanylyltransferase (Pseudomonas putida KT2440)
MMIPVILSGGSGSRLWPLSRKQFPKQFLALTGEHTLFQQTIERLVFEGMDTPIVVCNKDH
KFIVQEQLAALKLETQGILMEPFGRNTAPAVAMAAMKLVNEGRDELMLVLPADHVIDDQK
ALQRALALATVAAERGEMVLFGVPATKPETGYGYIRSSQDALLPEGVARVAQFVEKPDEK
RAAEFVQAGGYFWNSGMFLFRASRFLEELKKHDGDIYDTCVLALERSQEDGDVLSIDEAT
FACCPDNSIDYAVMEKTQRACVVPMSAGWSDVGCWSSLWEVHEKDDNGNVTKGDVVVQDS
RNCMIHGNGKLVSVIGLENIVVVETKDAMMIAHKDKVQGVKQMVKTLDEQGRTETQNHLE
VYRPWGSYDSVDMGGRFQVKHITVKPGASLSLQMHHHRAEHWIVVSGTAEVTCDENVFLL
TENQSTYIPIASVHRLRNPGKIPLEIIEVQSGSYLGEDDIERFEDVYGRTSTPIERGVSV
KTIAQ