Protein Info for PP_1271 in Pseudomonas putida KT2440

Annotation: putative Multidrug efflux MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 28 to 49 (22 residues), see Phobius details amino acids 69 to 89 (21 residues), see Phobius details amino acids 96 to 115 (20 residues), see Phobius details amino acids 124 to 148 (25 residues), see Phobius details amino acids 186 to 207 (22 residues), see Phobius details amino acids 219 to 240 (22 residues), see Phobius details amino acids 246 to 268 (23 residues), see Phobius details amino acids 288 to 313 (26 residues), see Phobius details amino acids 327 to 348 (22 residues), see Phobius details amino acids 355 to 374 (20 residues), see Phobius details amino acids 386 to 405 (20 residues), see Phobius details amino acids 422 to 442 (21 residues), see Phobius details amino acids 484 to 503 (20 residues), see Phobius details PF07690: MFS_1" amino acids 37 to 432 (396 residues), 107.6 bits, see alignment E=3.3e-35

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1271)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NE1 at UniProt or InterPro

Protein Sequence (513 amino acids)

>PP_1271 putative Multidrug efflux MFS transporter (Pseudomonas putida KT2440)
MTSLTAPSAALAAAPAPAAPAQTAFGLQVVVGLFGVLLAVLCAGLNESVTKISLADIRGA
MGIGADEGAWLLAVYSAASVSAMAFAPWLATTFSLRRFTMSAVGLFAVLGLLQPFAPNLH
SLMLLRVLQGFAAGALPPMLMSVALRFLPPGIKVYGLACYALTATFGPNLGTPLAGLWTE
YVGWQWAFWQIILPSLLAMVCVGWGLPQDPLRLERFKQFDWRGVLLGLPAISCTVLGLSL
GDRWGWFDSPLICWLLGGGLLLLVLFMYNEWSEPLPFFQLRLLQRRNLSFALVTLAGVLV
VLSGVGSIPSAYLAQIQGYRPAQTSPLMMLVAMPQLIALPLTAALCNIRAVDCRWVLGIG
LAMLAVSCVGSSLLTSEWIRGDFYPFYLLQVFGQPMAVLPLLMLSTNGMTPQEGPFASSW
FNTVKGLAAVIAGGLLDVLGTLRRHFHSNHLVDSLGNAPLVDGNAAGLAKRIHDQAQVLT
SADLYLVLACIAVALICLIPFVPTRVYPPRAVA