Protein Info for PP_1256 in Pseudomonas putida KT2440
Annotation: putative alpha-ketoglutarate semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 64% identical to KGSD3_AZOBR: Alpha-ketoglutaric semialdehyde dehydrogenase 3 (LhpG) from Azospirillum brasilense
KEGG orthology group: K14519, NADP-dependent aldehyde dehydrogenase [EC: 1.2.1.4] (inferred from 100% identity to ppu:PP_1256)MetaCyc: 100% identical to alpha-ketoglutaric semialdehyde dehydrogenase subunit (Pseudomonas putida KT2442)
2,5-dioxovalerate dehydrogenase. [EC: 1.2.1.26]
Predicted SEED Role
"Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Proline, 4-hydroxyproline uptake and utilization (EC 1.2.1.26)
MetaCyc Pathways
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- trans-4-hydroxy-L-proline degradation II (4/4 steps found)
- D-galactarate degradation II (3/3 steps found)
- D-glucarate degradation II (3/3 steps found)
- D-glucuronate degradation II (4/5 steps found)
- cytosolic NADPH production (yeast) (4/5 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- pyruvate fermentation to acetate VIII (1/2 steps found)
- D-galacturonate degradation II (3/5 steps found)
- 1,2-dichloroethane degradation (2/4 steps found)
- L-carnitine degradation II (1/3 steps found)
- L-lyxonate degradation (1/3 steps found)
- D-arabinose degradation III (3/6 steps found)
- L-arabinose degradation III (2/6 steps found)
- D-xylose degradation III (1/5 steps found)
- D-xylose degradation V (1/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)
- superpathway of pentose and pentitol degradation (10/42 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.26
Use Curated BLAST to search for 1.2.1.26 or 1.2.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88NF5 at UniProt or InterPro
Protein Sequence (525 amino acids)
>PP_1256 putative alpha-ketoglutarate semialdehyde dehydrogenase (Pseudomonas putida KT2440) MPLTGNLLIGQRPVTGSRDAIRAIDPTTGQTLEPAYLGGTGEHVAQACALAWAAFDAYRE TSLEQRAEFLEAIATQIEALGDALIDRAVIETGLPKARIQGERGRTCTQLRTFARTVRAG EWLDVRIDSALPERQPLPRADLRQRQVALGPVAVFGASNFPLAFSVAGGDTASALAAGCP VVVKAHSAHPGTSELVGQAVAQAVKQCGLPEGVFSLLYGSGREVGIALVSDPRIKAVGFT GSRSGGMALCQAAQARPEPIPVYAEMSSINPVFLFDAALQARAEALAQGFVASLTQGAGQ FCTNPGLVIARQGPALQRFITAAAGYVQQGAAQTMLTPGIFSAYQAGIAALADNPHAQAI TSGQAGQGPNQCQAQLFVTQAEAFLADPALQAEVFGAASLVVACTDDEQVRQVAEHLEGQ LTATLQLDEADIDSARALLPTLERKAGRILVNGWPTGVEVCDAMVHGGPFPATSDARTTS VGTAAILRFLRPVCYQDVPDALLPQALKHGNPLQLRRLLDGKRED