Protein Info for PP_1144 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 754 transmembrane" amino acids 26 to 44 (19 residues), see Phobius details amino acids 64 to 85 (22 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 125 to 147 (23 residues), see Phobius details amino acids 158 to 179 (22 residues), see Phobius details amino acids 199 to 220 (22 residues), see Phobius details amino acids 236 to 258 (23 residues), see Phobius details PF03707: MHYT" amino acids 73 to 122 (50 residues), 58.8 bits, see alignment 6.6e-20 amino acids 134 to 189 (56 residues), 47.7 bits, see alignment 1.8e-16 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 286 to 448 (163 residues), 157.4 bits, see alignment E=1.3e-50 PF00990: GGDEF" amino acids 290 to 445 (156 residues), 169.1 bits, see alignment E=1e-53 PF00563: EAL" amino acids 466 to 702 (237 residues), 253 bits, see alignment E=3.9e-79

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1144)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NR1 at UniProt or InterPro

Protein Sequence (754 amino acids)

>PP_1144 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MEWLGLQLFTDLPATGRIVIDCRHEPLLVLLAYFVACAACFATLDMAERQSHSDDPTAHR
QWNMLGACCLAGGIWAMHFISMLAFRAPVDVHYDVSLTILSLLIALGVAWLAMHSLERSN
MRAHYFVPSAGLIGLGIILMHFVGMAAMETGARQYYQTGLLLASAAIALLTALTALYLAR
YLRNGSGTLYQAMKYGASLLMAGGIVATHFTAMSAMTLVIPADTALRLPSGDNSLQLGLG
IGFITLLISGASISAALADKKLQSKEHDLRRVSVLLSQLDQARASLQQAAHYDALTNLLN
RRGFNQVFAERLVEHQASENRLAVMFLDIDHFKRINDSLGHDAGDELLKVIANHIKAATR
NHDLVARFGGDEFCVVTSLNSRDEARHLAQRIMQRMKDPIDLGGRRMVMTTSIGISIFPD
DGSTAEELLKHADLALYQSKDNGRNSLNFFNDSLKARASIALQLEEELRLALLEERGLCV
HYQPIFDLRSGQVAKLEALVRWQHPQHGLLGPDRFVGIAEANGLIIDLDLWVLRHACADL
AHLQRHGYGEVQVTVNCSAVTLSHDELPNEVEKALFHAGLAPRHLELEVTENALVGDIQR
TVSLLKRVRALGVALSIDDFGTGYSSLAYLKRLPLDVLKIDRTFLQDVPGSQKDREIVQA
IIAMAHTLHLRVVSEGVETAEQQAFLESHGCDYLQGYLLGRPVPLAELRPLLERLQRQNG
RITPCCGTALPGSPDLFAGNPGYRASASVARQGH