Protein Info for PP_1134 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 transmembrane" amino acids 101 to 121 (21 residues), see Phobius details PF21688: FAD-depend_C" amino acids 285 to 481 (197 residues), 275.5 bits, see alignment E=3.5e-86

Best Hits

Swiss-Prot: 52% identical to Y202_CLOB8: Uncharacterized protein Cbei_0202 (Cbei_0202) from Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052)

KEGG orthology group: K07137, (no description) (inferred from 100% identity to ppf:Pput_1169)

Predicted SEED Role

"NAD(FAD)-utilizing dehydrogenase, sll0175 homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NS1 at UniProt or InterPro

Protein Sequence (535 amino acids)

>PP_1134 conserved protein of unknown function (Pseudomonas putida KT2440)
MLRITELKLPLDHPDEALREAIVQRLGIRDEQLLSFNLFKRSYDARKKNSELLFIYTIDL
EASNEAELLSKFADDRNIGPAPDVTYKFVGQAPAGLQERPIVVGFGPCGIFAGLLLAQMG
FKPIILERGKEVRQRTKDTWGLWRKSVLNPESNVQFGEGGAGTFSDGKLYSQIKDPQHHG
RKVLEEFVKAGAPDEILYINKPHIGTFRLTGMVEQMRQDMIALGAEVRFQEKVTDLLIED
GQLTGVVLESGEQLHSRHVVLALGHSARDTFRMLHAKGVYMEAKPFSVGFRIEHPQTLID
KARLGKYAGHPKLGAADYKLVYHAKNGRSVYSFCMCPGGTVVAATSEPGRVVTNGMSQYS
RNERNANSGIVVGIDPERDYPGGPLAGIELQERLEAHAYVMGGSNYQAPAQLVGDFVAGR
PSTALGSVEPSYKPGVTLGDLAPSLPDFAIEAIREALPAFDRQIKGYNLHDAVLTGIETR
TSSPLRITRGEDYQSLNIKGLFPAGEGAGYAGGILSAGVDGIRIAEAVARDMLGL