Protein Info for PP_1079 in Pseudomonas putida KT2440

Annotation: Ornithine carbamoyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00658: ornithine carbamoyltransferase
  • amino acids 4 to 299 (296 residues), 371.4 bits, E=1.6e-115
• PF02729: OTCace_N
  • amino acids 4 to 144 (141 residues), 156.6 bits, E=5e-50
• PF00185: OTCace
  • amino acids 150 to 297 (148 residues), 174.7 bits, E=1.5e-55

Best Hits

Swiss-Prot: 100% identical to OTC_PSEPK: Ornithine carbamoyltransferase (argF) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00611, ornithine carbamoyltransferase [EC: 2.1.3.3] (inferred from 100% identity to ppu:PP_1079)

MetaCyc: 42% identical to ornithine carbamoyltransferase (Arabidopsis thaliana col)
Ornithine carbamoyltransferase. [EC: 2.1.3.3]

Predicted SEED Role

"Ornithine carbamoyltransferase (EC 2.1.3.3)" in subsystem Arginine Biosynthesis extended or Arginine Deiminase Pathway or Arginine and Ornithine Degradation (EC 2.1.3.3)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• urea cycle (3/5 steps found)
• L-arginine degradation XIV (oxidative Stickland reaction) (2/6 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.1.3.3

Use Curated BLAST to search for 2.1.3.3

Sequence Analysis Tools

See Q88NX4 at UniProt or InterPro

Protein Sequence (306 amino acids)

>PP_1079 Ornithine carbamoyltransferase (Pseudomonas putida KT2440)
MSARHFLSLLDFTTDELLGVIRHGIELKDLRKRGVLFEPLKNRVLGMIFEKSSTRTRVSF
EAGMIQLGGQAIFLSPRDTQLGRGEPIGDSAIVLSSMVDVVMIRTHAHSTLTEFAAKSRV
PVINGLSDESHPCQLLADMQTFVEHRGSIQGKTVTWIGDGFNMCNSYIEAARQFDFQLRI
ACPEGYEPDQRFMALGGDRVQIIRDAREAVRDAHLVVTDVWTSMGQEEETARRLAHFAPY
QVTRELLDLAAPDVLFMHCLPAHRGEEISQDLLDDPRSVAWDEAENRLHAQKALLEFLVE
PAYHHA