Protein Info for PP_1077 in Pseudomonas putida KT2440

Annotation: Cys-tRNAPro deacylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 156 TIGR00011: Cys-tRNA(Pro) deacylase" amino acids 2 to 153 (152 residues), 181.3 bits, see alignment E=5.1e-58 PF04073: tRNA_edit" amino acids 32 to 142 (111 residues), 85.7 bits, see alignment E=1.4e-28

Best Hits

Swiss-Prot: 59% identical to YBAK_ECOLI: Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK (ybaK) from Escherichia coli (strain K12)

KEGG orthology group: K03976, putative transcription regulator (inferred from 100% identity to ppf:Pput_1118)

MetaCyc: 59% identical to Cys-tRNAPro/Cys-tRNACys deacylase YbaK (Escherichia coli K-12 substr. MG1655)
3.1.1.M26 [EC: 3.1.1.M26]

Predicted SEED Role

"Cys-tRNA(Pro) deacylase YbaK"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.M26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NX6 at UniProt or InterPro

Protein Sequence (156 amino acids)

>PP_1077 Cys-tRNAPro deacylase (Pseudomonas putida KT2440)
MTPALDLLKKHRAEHRVHSYEHDPKSASYGLEAAEKLGLDPQQVFKTLLASSEKGELLVA
VVPVVGTLDLKALAHAAGVKKCEMADPAAAQRATGYLVGGISPLGQKKRLRTFIDDSAQN
FATIHVSAGRRGLEVELAAAVLAEYTQGTFAGIGRG