Protein Info for PP_1024 in Pseudomonas putida KT2440

Annotation: KHG/KDPG aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01081: Aldolase
  • amino acids 29 to 224 (196 residues), 316.4 bits, E=3.1e-99
• TIGR01182: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase
  • amino acids 30 to 230 (201 residues), 259.1 bits, E=1.1e-81

Best Hits

Swiss-Prot: 98% identical to ALKD_PSEPU: 2-dehydro-3-deoxy-phosphogluconate aldolase (eda) from Pseudomonas putida

KEGG orthology group: K01625, 2-dehydro-3-deoxyphosphogluconate aldolase / 4-hydroxy-2-oxoglutarate aldolase [EC: 4.1.2.14 4.1.3.16] (inferred from 100% identity to ppu:PP_1024)

MetaCyc: 50% identical to 2-dehydro-3-deoxy-D-gluconate-6-phosphate aldolase (Dickeya dadantii 3937)
2-dehydro-3-deoxy-phosphogluconate aldolase. [EC: 4.1.2.14, 4.1.2.55]

Predicted SEED Role

"4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14)" (EC 4.1.2.14, EC 4.1.3.16)

MetaCyc Pathways

• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• Entner-Doudoroff pathway I (8/9 steps found)
• Entner-Doudoroff shunt (2/2 steps found)
• trans-4-hydroxy-L-proline degradation I (3/5 steps found)
• D-glucosaminate degradation (1/3 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (5/9 steps found)
• D-galacturonate degradation I (2/5 steps found)
• D-fructuronate degradation (1/4 steps found)
• 4-deoxy-L-threo-hex-4-enopyranuronate degradation (1/5 steps found)
• 3,6-anhydro-α-L-galactopyranose degradation (2/7 steps found)
• superpathway of β-D-glucuronosides degradation (2/7 steps found)
• superpathway of hexuronide and hexuronate degradation (3/10 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glyoxylate and dicarboxylate metabolism
• Pentose and glucuronate interconversions
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.14 or 4.1.2.55 or 4.1.3.16

Sequence Analysis Tools

See Q88P29 at UniProt or InterPro

Protein Sequence (235 amino acids)

>PP_1024 KHG/KDPG aldolase (Pseudomonas putida KT2440)
MPMSQGSTVMTTLERPQPKLSMADKAARIDAICKKARILPVITIAREEDILPLADALAAG
GIRTLEVTLRSQHGLKAIQVLREQRPELCVGAGTVLDRSMFAAVEAAGAQFVVTPGITQD
ILEAGVDSEIPLLPGISTPSEIMMGYALGYRRFKLFPAEISGGVAAIKAFGGPFGDIRFC
PTGGVNPANVRNYMALPNVMCVGGTWMLDSSWIKNGDWARIEACSAEAMALLDAN