Protein Info for PP_0995 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 transmembrane" amino acids 27 to 47 (21 residues), see Phobius details amino acids 59 to 78 (20 residues), see Phobius details amino acids 90 to 113 (24 residues), see Phobius details amino acids 130 to 149 (20 residues), see Phobius details amino acids 159 to 178 (20 residues), see Phobius details amino acids 188 to 207 (20 residues), see Phobius details PF06532: NrsF" amino acids 10 to 213 (204 residues), 247.5 bits, see alignment E=5.7e-78

Best Hits

Swiss-Prot: 50% identical to NRSF_AZOOP: Probable anti-sigma-F factor NrsF (nrsf) from Azospira oryzae (strain ATCC BAA-33 / DSM 13638 / PS)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0995)

Predicted SEED Role

"EXTRACYTOPLASMIC FUNCTION ALTERNATIVE SIGMA FACTOR"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P58 at UniProt or InterPro

Protein Sequence (213 amino acids)

>PP_0995 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MKTDDLIALLAAGEGPVPRHAVGRRMAVAALGGLTAALLLTITLYGVRSDITEVAQTPLF
WGKVAFPTSLALIGLWLTSRLARPGGKGAAGWKMLGLPLLLVWCGAAVSIAGAPVDARAD
LLFGRTWRTCALNIALLSVPAFVTVFWALKGLAPTRLRLAGAAGGLLAGSSATVAYCLHC
PEMGVPFWGVWYVLGMLVPTVLGAWWGPRMLRW