Protein Info for PP_0979 in Pseudomonas putida KT2440

Annotation: DNA polymerase III subunit chi

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 142 PF04364: DNA_pol3_chi" amino acids 1 to 135 (135 residues), 139.2 bits, see alignment E=5.1e-45

Best Hits

Swiss-Prot: 66% identical to HOLC_PSEAE: DNA polymerase III subunit chi (holC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02339, DNA polymerase III subunit chi [EC: 2.7.7.7] (inferred from 100% identity to ppu:PP_0979)

Predicted SEED Role

"DNA polymerase III chi subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P74 at UniProt or InterPro

Protein Sequence (142 amino acids)

>PP_0979 DNA polymerase III subunit chi (Pseudomonas putida KT2440)
MSKVDFYILPTDSLSARLDFACKLCEKAWRLGHRVYLHCQDAEQRSELDQRLWRFKGEAF
VPHDLAEVHADADVVLGLADNAGEHHGLLINLGASVPGFVGQFERVAEIVVEEPAIRQSA
RERFRFYREQGYALQDHRLQRL