Protein Info for PP_0902 in Pseudomonas putida KT2440

Annotation: integral membrane sensor signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 131 to 155 (25 residues), see Phobius details PF00672: HAMP" amino acids 153 to 207 (55 residues), 28.3 bits, see alignment 2.8e-10 PF00512: HisKA" amino acids 213 to 275 (63 residues), 52.1 bits, see alignment E=8.4e-18 PF02518: HATPase_c" amino acids 324 to 423 (100 residues), 71.2 bits, see alignment E=1.5e-23

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_0941)

Predicted SEED Role

"two-component system sensor protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PE8 at UniProt or InterPro

Protein Sequence (436 amino acids)

>PP_0902 integral membrane sensor signal transduction histidine kinase (Pseudomonas putida KT2440)
MEFKQSLAQRIIIAFALMSALVAGAFAFGIVGTVHLVEERLISSVLGGDLQRLLRMDSVS
DWSHRPRPDQLFYFSGGRDDFELPKDLRHLDRGFHEVFRDQLSYHAMVEIVDGRRYVLLQ
DQSDFEERERVLFAVVVVGFVLSLVLAVILGWLVARRVMAPVIRLARQVRHRDQLLGLAP
PLAPDYAADEVGQLAVAFDDTLGRLRDALTRERLFTSDVSHELRTPLMVLATSCELLMEN
PGLDARARSQVERVARATEEMRELVKTFLMLARAQRDEGAVASRATLREVADELIGVWRD
TIEQKGLTLYFDGRVSASPVLYNATFLQSVMGNLLRNAAHYTDSGYIRLSLEANGFSVED
SGVGIPEEQREAMFRPFVRGDERRGEGLGLGLSLVQRICDDQGWRVTLTSTLPHGCRFQV
DLSNTATKGDPDALVE