Protein Info for PP_0901 in Pseudomonas putida KT2440

Annotation: DNA-binding response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 227 PF00072: Response_reg" amino acids 3 to 114 (112 residues), 107 bits, see alignment E=6.3e-35 PF00486: Trans_reg_C" amino acids 149 to 220 (72 residues), 64.2 bits, see alignment E=9.5e-22

Best Hits

Swiss-Prot: 41% identical to CZCR_CUPMC: Transcriptional activator protein CzcR (czcR) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: None (inferred from 100% identity to ppw:PputW619_0969)

Predicted SEED Role

"DNA-binding response regulator ColR"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PE9 at UniProt or InterPro

Protein Sequence (227 amino acids)

>PP_0901 DNA-binding response regulator (Pseudomonas putida KT2440)
MRILLVEDNRDILANLADYLGMKGYTVDCAQDGLSGLHLAATEHYDLIVLDIMLPGIDGY
TLCKRLREDARRDTPVIMLTARDQLDDRLQGFRSGADDYLLKPFALSELAARIEAVLRRA
QGGGRRTLQVADLSYDLDTLEVTRQGRLLKLNPVGLKLLAVLMQKSPHVLRREVLEEALW
GDDCPDSDSLRSHVHQLRQVIDKPFEKPLLHTVHGVGYRLAEGRDGV