Protein Info for PP_0878 in Pseudomonas putida KT2440

Annotation: dipeptide ABC transporter, binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 PF00005: ABC_tran" amino acids 30 to 182 (153 residues), 125.4 bits, see alignment E=3.9e-40 PF08352: oligo_HPY" amino acids 234 to 298 (65 residues), 68.6 bits, see alignment E=7.4e-23 TIGR01727: oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain" amino acids 234 to 317 (84 residues), 89.6 bits, see alignment E=5.6e-30

Best Hits

Swiss-Prot: 50% identical to Y4TS_SINFN: Probable peptide ABC transporter ATP-binding protein y4tS (NGR_a01400) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K12372, dipeptide transport system ATP-binding protein (inferred from 99% identity to ppg:PputGB1_0921)

MetaCyc: 48% identical to murein tripeptide ABC transporter / oligopeptide ABC transporter ATP binding subunit OppF (Escherichia coli K-12 substr. MG1655)
3.6.3.23-RXN [EC: 7.4.2.6]; 7.4.2.6 [EC: 7.4.2.6]

Predicted SEED Role

"Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2)" in subsystem ABC transporter dipeptide (TC 3.A.1.5.2) (TC 3.A.1.5.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PH2 at UniProt or InterPro

Protein Sequence (322 amino acids)

>PP_0878 dipeptide ABC transporter, binding subunit (Pseudomonas putida KT2440)
MAVVLSARELTRHYEVSRGLFKGHALVRALNGVSFELEAGKTLAVVGESGCGKSTLARAL
TLIEEPSSGSLQIAGTEVKGASKAERKQLRRDVQMVFQSPYASLNPRQKIGDQLAEPLLI
NTSLSKAERRDKVQKMMEQVGLRPEHYQRYPHMFSGGQRQRIALARAMMLQPKVLVADEP
TSALDVSIQAQVLNLFMDLQKEFNTAYVFISHNLAVVRHVADQVLVMYLGRPAEMGPKED
IYAKPLHPYTQALLSATPAIHPDPLKPKIRIVGELPNPLNPPDGCAFHKRCPYATERCAK
EVPALRQVSTRQVACHYAEQFL