Protein Info for PP_0868 in Pseudomonas putida KT2440

Annotation: ABC transporter ATP-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 TIGR01186: glycine betaine/L-proline transport ATP binding subunit" amino acids 19 to 364 (346 residues), 347.9 bits, see alignment E=3.3e-108 PF00005: ABC_tran" amino acids 22 to 167 (146 residues), 126 bits, see alignment E=2.5e-40 PF00571: CBS" amino acids 259 to 303 (45 residues), 30 bits, see alignment 8.3e-11

Best Hits

Swiss-Prot: 62% identical to OSMV_SALTY: Osmoprotectant import ATP-binding protein OsmV (osmV) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K05847, osmoprotectant transport system ATP-binding protein (inferred from 100% identity to ppf:Pput_0898)

Predicted SEED Role

"Glycine betaine/carnitine/choline ABC transporter, ATP-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PI2 at UniProt or InterPro

Protein Sequence (385 amino acids)

>PP_0868 ABC transporter ATP-binding subunit (Pseudomonas putida KT2440)
MIELKNLSKTFNVNGKDVKAVDSVSLTVNEGEICVFLGPSGCGKSTTLKMINRLITPTSG
QVFINGEDTTGLDEVTLRRHIGYVIQQIGLFPNMTIEENITVVPRLLGWDKQKCHERARE
LMHMIKLEPKQYLQRYPRELSGGQQQRIGVIRALAADAPVLLMDEPFGAVDPINREMIQN
EFFEMQRALNKTVIMVSHDIDEAIKLGDKIAIFRAGKLLQLDHPDTLLAHPADDFVSNFV
GQDSTLKRLLLVRAEDAADNAPSVSPETPVGDALELLDEHDRRYVVVTDAQNKALGYVRR
RDMHRQQGTCGDFLRPFNATASHDEHLRILLSRMYEFNRAWLPVLDAEQVFLGEVTQESI
AAYLSSGRSRGAKSSIVSPAEAVVS