Protein Info for PP_0854 in Pseudomonas putida KT2440

Annotation: Histidine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 TIGR00442: histidine--tRNA ligase" amino acids 6 to 414 (409 residues), 505.8 bits, see alignment E=4.7e-156 PF13393: tRNA-synt_His" amino acids 10 to 313 (304 residues), 142.8 bits, see alignment E=2.2e-45 PF00587: tRNA-synt_2b" amino acids 68 to 317 (250 residues), 90.4 bits, see alignment E=2.2e-29 PF03129: HGTP_anticodon" amino acids 335 to 422 (88 residues), 28.7 bits, see alignment E=1.8e-10

Best Hits

Swiss-Prot: 100% identical to SYH_PSEPK: Histidine--tRNA ligase (hisS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 100% identity to ppu:PP_0854)

MetaCyc: 63% identical to histidine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Histidine--tRNA ligase. [EC: 6.1.1.21]

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PJ6 at UniProt or InterPro

Protein Sequence (429 amino acids)

>PP_0854 Histidine--tRNA ligase (Pseudomonas putida KT2440)
MSKSLQAIRGMNDILPEQSPLWRYFEGTVAGLLDTYGYSQIRTPIVEFTELFKRSIGEVT
DIVEKEMYTFEDRNGDSLTLRPEGTAACVRAVLEHGITGNGQVQKLWYIGQMFRHERPQK
GRYRQFHQIGVEVFNLDGPDIDAELIMLTWRLWGLLGIQDAVKLELNSLGTSEARARYRD
ALVEFLSARLEQLDEDSQRRLKSNPLRILDSKDQNTQAVLVGAPKLEDYLDEESRVHFEG
LKARLDAAGIPFVINTKLVRGLDYYSKTVFEWVTDKLGAQGTVCAGGRYDGLVEQMGGKP
TTGVGFAMGIERLILLLETLGKVPESISRQIDVYLCAFGEQAELAGLRLSEGLRDRLPGL
RLAVNAGGGSFKSQFKKADKSGALFALILGDDELAKQEIGLKPLRGQGEQQNIAWDALAE
HLETAIAQA