Protein Info for PP_0842 in Pseudomonas putida KT2440

Annotation: cysteine desulfurase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02006: cysteine desulfurase IscS
  • amino acids 1 to 404 (404 residues), 744.7 bits, E=1.3e-228
• PF00266: Aminotran_5
  • amino acids 5 to 368 (364 residues), 289.5 bits, E=4e-90
• PF01041: DegT_DnrJ_EryC1
  • amino acids 69 to 187 (119 residues), 20 bits, E=3.6e-08

Best Hits

Swiss-Prot: 100% identical to ISCS_PSEPK: Cysteine desulfurase IscS (iscS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 100% identity to ppg:PputGB1_0885)

MetaCyc: 72% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of L-alanine biosynthesis (4/4 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• molybdopterin biosynthesis (4/6 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (4/7 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (3/6 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (4/10 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (4/11 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

See Q88PK8 at UniProt or InterPro

Protein Sequence (404 amino acids)

>PP_0842 cysteine desulfurase (Pseudomonas putida KT2440)
MKLPIYLDYSATTPVDPRVAQKMADCLLVDGNFGNPASRSHVFGWKAEEAVENGRRQVAE
LINADPREIVWTSGATESDNLALKGVAHFYQTKGKHIITSKIEHKAVLDTARQLEREGFE
VTYLEPGEDGIVTPAMVEAVLRDDTILVSLMHVNNEVGSINDIAAIGELTRSRGVLFHVD
AAQSAGKVEIDLQKLKVDLMSFSAHKVYGPKGIGALYVSRKPRVRLEAIIHGGGHERGMR
SGTLPTHQIVGMGEAFAIAKQEMVAENARIKALSDRFFKQVSNLEELYVNGSQTARVPHN
LNLSFNYVEGESLLMSLKDIAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFSFGRF
TTEEEVDYAAQKVCEAVNKLRELSPLWDMYKDGVDISKIEWAAH