Protein Info for PP_0836 in Pseudomonas putida KT2440

Annotation: Sec translocation complex subunit SecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details amino acids 129 to 146 (18 residues), see Phobius details amino acids 153 to 175 (23 residues), see Phobius details amino acids 181 to 202 (22 residues), see Phobius details amino acids 228 to 248 (21 residues), see Phobius details amino acids 255 to 282 (28 residues), see Phobius details PF07549: Sec_GG" amino acids 26 to 53 (28 residues), 40 bits, see alignment (E = 2e-14) TIGR00966: protein-export membrane protein SecF" amino acids 32 to 275 (244 residues), 269.2 bits, see alignment E=3.9e-84 TIGR00916: protein-export membrane protein, SecD/SecF family" amino acids 93 to 272 (180 residues), 190.1 bits, see alignment E=4.4e-60 PF02355: SecD_SecF_C" amino acids 108 to 283 (176 residues), 222.4 bits, see alignment E=3.5e-70

Best Hits

Swiss-Prot: 77% identical to SECF_PSEAE: Protein translocase subunit SecF (secF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03074, preprotein translocase subunit SecF (inferred from 100% identity to ppu:PP_0836)

MetaCyc: 46% identical to Sec translocon accessory complex subunit SecF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Protein-export membrane protein SecF (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PL4 at UniProt or InterPro

Protein Sequence (302 amino acids)

>PP_0836 Sec translocation complex subunit SecF (Pseudomonas putida KT2440)
MKTINFMGVRNVAFAVTVLLTVLALFSWWQKGLNFGLDFTGGTLIELTYERPADVKAVRA
ELVESGFNEAVVQSFGATTDLLVRMPGDDPMLGNKVAEALQKAGGDNPAKVKRVEFVGPQ
VGEELRDQGGIGMLLALGGILIYLAFRFQWKFAVGAIVSLIHDVVVTLGILSFFQITFDL
TVLAAVLAIIGYSLNDTIVVFDRVRENFRVMRKASLIENINVSTTQTLLRTVATSVSTLL
AIAALLFFGGDNLFGFSLALFIGVMAGTYSSIYIANVVLIWLNLSSEDLIPPVKAEGVDE
RP