Protein Info for PP_0822 in Pseudomonas putida KT2440

Annotation: tRNA 2-selenouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 TIGR03167: tRNA 2-selenouridine synthase" amino acids 16 to 348 (333 residues), 363.1 bits, see alignment E=7e-113

Best Hits

Swiss-Prot: 100% identical to SELU_PSEPK: tRNA 2-selenouridine/geranyl-2-thiouridine synthase (selU) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K06917, tRNA 2-selenouridine synthase [EC: 2.9.1.-] (inferred from 100% identity to ppf:Pput_0849)

MetaCyc: 46% identical to tRNA 2-selenouridine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-2281 [EC: 2.9.1.3]

Predicted SEED Role

"Selenophosphate-dependent tRNA 2-selenouridine synthase" in subsystem Selenocysteine metabolism

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.9.1.- or 2.9.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PM7 at UniProt or InterPro

Protein Sequence (370 amino acids)

>PP_0822 tRNA 2-selenouridine synthase (Pseudomonas putida KT2440)
MRPDCTDFRQLFLDDVPMMDMRAPVEFAKGAFPGVVNLPLMNDQERQKVGTCYKQQGQAA
AIALGHQLVSGATKQARLEAWAAFAKAHPDGYLYCFRGGLRSQIVQGWLRDEAGIQYPRV
KGGYKAMRTFLLETTQQAVEQCDFVLVGGLTGTGKTDVLHQLDNVLDLEGHANHRGSSFG
KRATAQPAQIDFENQLAIDVLKKRARGIGQFVLEDEGRIVGSCTVPLELYQGMQHYPLVW
LEDSFTNRVERILRDYVVNLSAEFKAVHGEEDGPRLFAERMLQSMANIYKRLGGERHQRL
SEMLREALQEQQRSGAVDLHRGWIEGLLNEYYDPMYAYQRAAKAERIEFAGDAVEVREYL
KARALRQPRK