Protein Info for PP_0793 in Pseudomonas putida KT2440

Annotation: Phosphotransferase system, fructose-specific EI/HPr/EIIA components

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 PF00359: PTS_EIIA_2" amino acids 5 to 140 (136 residues), 100.3 bits, see alignment E=2.6e-32 PF00381: PTS-HPr" amino acids 285 to 366 (82 residues), 61.5 bits, see alignment E=1.7e-20 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 400 to 948 (549 residues), 481.8 bits, see alignment E=2.6e-148 PF05524: PEP-utilisers_N" amino acids 401 to 516 (116 residues), 80.5 bits, see alignment E=3e-26 PF00391: PEP-utilizers" amino acids 542 to 613 (72 residues), 77 bits, see alignment 1.7e-25 PF02896: PEP-utilizers_C" amino acids 647 to 923 (277 residues), 360.6 bits, see alignment E=1.4e-111

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to ppu:PP_0793)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) / Fructose-specific phosphocarrier protein HPr (EC 2.7.1.69) / PTS system, fructose-specific IIA component (EC 2.7.1.69)" in subsystem Fructose utilization or Fructose and Mannose Inducible PTS or Mannitol Utilization (EC 2.7.1.69, EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PQ5 at UniProt or InterPro

Protein Sequence (950 amino acids)

>PP_0793 Phosphotransferase system, fructose-specific EI/HPr/EIIA components (Pseudomonas putida KT2440)
MLELANEQIAMGQKAADKAEALRLLADRLVADGLVAEGYLQGLQAREAQGSTFLGQGIAI
PHGTPQTRDLVYATGVRLLQFPEGVDWGDGQMVYLAIGIAARSDEHLRLLQLLTRALGET
DLAEALRRASSAEALLKLLQGAPQALALDAQLVGLNVPAEDFDELAWRGARLLQRADCVD
SGFAAVLQQAEPLPLGEGLWWLHSERQVRQPGLAFITPQQPLRYRDQPLNGLFCLASLGA
AHEALLERLCEVLIEGRGQVLYQATSSRAVLEVLGGEVPADWPSARVVLANPHGLHARPA
KVLAQLAKGFEGEIRVRLVDSAQPAVSVKSLSKLLSLGARRGQALELVAEPSIAADALPV
LLAAIEQGLGEEVEPLPQSVAPIADDVPEVLQAPAAGSRIQGVGAAPGIASGPAHVCVER
EFDYPLRGESCALERQKLREALATVNGELQALVLRSDKAIGEIFVTHQEMLADPALTDDV
EQRLAQGESAAAAWMAVIEAAARQQEALHDALLAERAADLRDIGRRVLAQLCGVQAQVEP
EQPYVLVMTEVGPSDVARLDPNRVAGIVTAQGGATAHSAIVARALGIPAVVGAGASILLL
ESGTPLLLDGQRGVVSVAPPADELQRALAERDLREQRLQAAWANRFEPAITRDGHAVEVF
ANIGDSNGIAKVVEQGAEGVGLLRTELIFMAHPQAPDVATQEAEYRRVLDGLDGRPLVVR
TLDVGGDKPLPYWPIAAEENPFLGVRGVRLTLQRPQVMEDQLRALLRAADQRPLRIMFPM
VGQVHEWREARAMVERLRAEIPVADLQLGIMVEVPSAALLAAQLAREVDFFSIGTNDLTQ
YTLAIDRGHPSLSAQADGLHPAVLSLIDMTVRAAHAHGKWVGVCGELAADPQAVAVLLGL
DVDELSVSARSIAEVKALVRQADHQTARALAREALQQDSAAAVRALVERY