Protein Info for PP_0766 in Pseudomonas putida KT2440

Updated annotation (from data): adhesin-associated sorting protein (DUF1329)
Rationale: Conserved cofit with a putative adhesin (PP_0765). DUF1329 is distantly related to lolB, which inserts lipoproteins into the outer membrane. So we predict that DUF1329 is involved in sorting the adhesin to the outer membrane.
Original annotation: conserved exported protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF07044: DUF1329" amino acids 84 to 449 (366 residues), 529.9 bits, see alignment E=2e-163

Best Hits

Swiss-Prot: 78% identical to Y3922_PSEAE: Uncharacterized protein PA3922 (PA3922) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_0766)

Predicted SEED Role

"FIG002188: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PT2 at UniProt or InterPro

Protein Sequence (454 amino acids)

>PP_0766 adhesin-associated sorting protein (DUF1329) (Pseudomonas putida KT2440)
MNKTRSLLQAGVLGLSLLATSVMAAVSADEAAKLGSTLTPMGAEKAGNADGSIGPWEPLS
KTAGSVDGKGFLSDPYGSEKPLFTITAQNADQYKDKLSPGQLAMFKRYPNTYKIPVFKTH
RGATVPADVFAAIKENATKTTLVEGGNGLSNFRTAVPFPIPKSGLEVIWNHITRYRGGSV
SRLVTQATPQQNGSFNPVYFSDQFVFRDKMKDYDPNNPGNILFYFKQEVTAPARLAGTVL
LVHETLDQVKEPRKAWIYNAGQRRVRQAPQVSYDGPGTAADGLRTSDNLDMFNGAPDRYD
WKLEGKKELYIASNAFKLDDPKLKYADIIKAGHINQDLARYELRRVWHVVATLKPGQRHI
YAKRDFYIDEDTWQAAVIDQYDGRGQLWRVSEAHAQPYYNVEVPWYTLEAIYDLQSGRYL
ALGMKNEEKRAYDFGFSASKADFQPAALRQSGIR