Protein Info for PP_0745 in Pseudomonas putida KT2440

Annotation: putative uracil permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 39 to 55 (17 residues), see Phobius details amino acids 62 to 81 (20 residues), see Phobius details amino acids 87 to 104 (18 residues), see Phobius details amino acids 116 to 137 (22 residues), see Phobius details amino acids 155 to 173 (19 residues), see Phobius details amino acids 180 to 200 (21 residues), see Phobius details amino acids 223 to 248 (26 residues), see Phobius details amino acids 303 to 324 (22 residues), see Phobius details amino acids 330 to 352 (23 residues), see Phobius details amino acids 364 to 383 (20 residues), see Phobius details amino acids 389 to 408 (20 residues), see Phobius details TIGR00801: uracil-xanthine permease" amino acids 9 to 407 (399 residues), 370.4 bits, see alignment E=6.2e-115 PF00860: Xan_ur_permease" amino acids 12 to 378 (367 residues), 318.9 bits, see alignment E=2e-99

Best Hits

Swiss-Prot: 59% identical to URAA_PASMU: Probable uracil permease (uraA) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K02824, uracil permease (inferred from 100% identity to ppf:Pput_0773)

MetaCyc: 40% identical to uracil:H+ symporter UraA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-132

Predicted SEED Role

"Uracil permease" in subsystem De Novo Pyrimidine Synthesis or Pyrimidine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PV3 at UniProt or InterPro

Protein Sequence (421 amino acids)

>PP_0745 putative uracil permease (Pseudomonas putida KT2440)
MQDGFNDPLWRQVVSGAQMLFVAFGALVLMPLITGLDPNVALFTAGIGTLLFQLVTGRQV
PVFLASSFAFITPIILAKGQFGLAETMGGVMAAGFVYTFMGLMVKIKGTGFIDRMLPPVV
IGPVIISIGLAMAPIAANMAMGKGGDGTALMPYKTAMLISMPALLTTLIVAVFGKGIFRL
VPIISGVLVGFALSFAFGVVDTARIAAAPWLEIPNFTAPAFNWQAILFIVPVALAPAIEH
IGGVIAVGSVTGRDYLKKPGLHRTLLGDGLATTAAGLFGGPPNTTYAEVTGAVMLTKNYN
PKIMTWAAVFAITLAFIGKFGALLQSIPVPVMGGILCLLFGSIAAVGMNTMIRHKIDLGE
ARNLVIVSVTLVFGIGGVLIGSGDGPDDWGLKGIALCAIVAIALNLILPGNDSWKHKKLD
V