Protein Info for PP_0743 in Pseudomonas putida KT2440

Annotation: conserved protein with NAD(P)-binding Rossmann-fold domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 301 PF01370: Epimerase" amino acids 3 to 223 (221 residues), 119.1 bits, see alignment E=3.3e-38 TIGR01777: TIGR01777 family protein" amino acids 3 to 294 (292 residues), 355.7 bits, see alignment E=1.1e-110 PF08338: DUF1731" amino acids 251 to 297 (47 residues), 70.3 bits, see alignment 1.4e-23

Best Hits

Swiss-Prot: 41% identical to Y1208_HAEIN: Epimerase family protein HI_1208 (HI_1208) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K07071, (no description) (inferred from 100% identity to ppu:PP_0743)

Predicted SEED Role

"Cell division inhibitor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PV5 at UniProt or InterPro

Protein Sequence (301 amino acids)

>PP_0743 conserved protein with NAD(P)-binding Rossmann-fold domain (Pseudomonas putida KT2440)
MHILLTGGTGLIGKHLCQYWLGQGHRLTVWSRRPEQVAKICGSGVRGIARLEQLEADDHL
DAVVNLAGAPIADRPWTAARRNVLWASRVTLTEQLLAWLGTREQRPEVLISGSAVGWYGD
GGERELTEASPPVREDFASQLCIAWEETAQRSQAQGIRVVLVRTGLVLASDGGFLSRLRM
PFKLGLGGPLGNGRQWMPWVHIDDQVALIDFLLQHKDASGPYNACAPEPVRNREFARRLG
RALHRPALLPVPALLLKAGLGEMSTLLLGGQRARPVRLLEAGFTFRFNDLQSALDNLSSR
L