Protein Info for PP_0734 in Pseudomonas putida KT2440

Annotation: release factor-(glutamine-N5) methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 PF17827: PrmC_N" amino acids 12 to 72 (61 residues), 64.8 bits, see alignment E=4.4e-21 TIGR00536: methyltransferase, HemK family" amino acids 17 to 275 (259 residues), 240.7 bits, see alignment E=1.7e-75 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 22 to 270 (249 residues), 298.8 bits, see alignment E=3.3e-93 PF05175: MTS" amino acids 94 to 190 (97 residues), 61 bits, see alignment E=5.8e-20 PF06325: PrmA" amino acids 101 to 182 (82 residues), 27.3 bits, see alignment E=1.3e-09 PF13489: Methyltransf_23" amino acids 106 to 237 (132 residues), 30.6 bits, see alignment E=1.3e-10 PF03848: TehB" amino acids 107 to 182 (76 residues), 23.4 bits, see alignment E=1.8e-08 PF03602: Cons_hypoth95" amino acids 108 to 188 (81 residues), 26.7 bits, see alignment E=2.1e-09 PF13847: Methyltransf_31" amino acids 110 to 193 (84 residues), 51.4 bits, see alignment E=4.7e-17 PF13649: Methyltransf_25" amino acids 112 to 183 (72 residues), 39.1 bits, see alignment E=5.2e-13 PF08242: Methyltransf_12" amino acids 113 to 182 (70 residues), 31.6 bits, see alignment E=1.1e-10 PF08241: Methyltransf_11" amino acids 113 to 182 (70 residues), 28.6 bits, see alignment E=9.5e-10

Best Hits

Swiss-Prot: 71% identical to PRMC_PSEAE: Release factor glutamine methyltransferase (prmC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to ppu:PP_0734)

MetaCyc: 49% identical to protein-(glutamine-N5) methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-14992 [EC: 2.1.1.297]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.297

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PW4 at UniProt or InterPro

Protein Sequence (276 amino acids)

>PP_0734 release factor-(glutamine-N5) methyltransferase (Pseudomonas putida KT2440)
MTVIASLLRNAQLPESPTERLDAELLLAAAIGKSRSYLHTWPERIVSSEDAETYAGYLKR
RRGGEPVAYILGLQGFWKIDLEVAPHTLIPRPDTELLVETALELQPASPAKVLDLGTGTG
AIALALASERPAWQVTAVDRVEEAAALAERNRQRLGLANARVRLSHWFDSLAAERFDLIV
SNPPYIAAEDPHLVAGDVRFEPSSALVAGADGLDDLRVIAAQAPAHLLPGGWLLLEHGYD
QAAAVRALLAEHGFIEVASRTDLGGHERITLGRLSC