Protein Info for PP_0731 in Pseudomonas putida KT2440

Annotation: phosphatidylcholine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 611 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 43 to 65 (23 residues), see Phobius details amino acids 76 to 95 (20 residues), see Phobius details amino acids 101 to 118 (18 residues), see Phobius details amino acids 136 to 158 (23 residues), see Phobius details amino acids 165 to 185 (21 residues), see Phobius details amino acids 219 to 244 (26 residues), see Phobius details amino acids 250 to 271 (22 residues), see Phobius details amino acids 281 to 300 (20 residues), see Phobius details amino acids 306 to 323 (18 residues), see Phobius details amino acids 336 to 358 (23 residues), see Phobius details amino acids 364 to 385 (22 residues), see Phobius details amino acids 419 to 440 (22 residues), see Phobius details amino acids 446 to 466 (21 residues), see Phobius details amino acids 487 to 504 (18 residues), see Phobius details amino acids 510 to 529 (20 residues), see Phobius details amino acids 539 to 558 (20 residues), see Phobius details amino acids 562 to 581 (20 residues), see Phobius details amino acids 588 to 609 (22 residues), see Phobius details PF01066: CDP-OH_P_transf" amino acids 422 to 565 (144 residues), 55.3 bits, see alignment E=5.3e-19

Best Hits

Predicted SEED Role

"FIG00973969: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PW7 at UniProt or InterPro

Protein Sequence (611 amino acids)

>PP_0731 phosphatidylcholine synthase (Pseudomonas putida KT2440)
MEEFAVSRRHSVLLWGVNLCAISASTLSYIYFSYYIYAHTASLILSQVVLFAPMVLPVIL
VAPIYRLADYLAPRTLLWSANLLSLGCAAATYKILPHHVEVVVLGAVVIGTLDALQRVGR
IVAIKCYFPGAQIASSVPLTLTAQFIAGGLAGISLLLFKANMTPGLALLITVSLFSIAAL
AAYLLPAPTKPPITVQVAPRLLTALGSLLHEYPALRLSLYQFIVFVSLFQGFFNVSRVLL
PAYVLGLGEVYVGLLQAVNSVAALVGAVLFYRLGKCNVQPLPMPMAVVSGLFMMLAAFGH
DIQSSYIAYFLYIFFFELAFFKLQADLVRSTPTSAMPLMASVQYAGVYLGMIITIFAGSI
LVKYVGLFSTSIIFVAFYFVASNALRTTVRDQGKTTCLNNFFKENNMPTLARVFPFVRLL
DLANILTMFNIGLAFGTVYFALQRRFVLAATMICLAAMLDFLDGHIARRYLAANSAQRAF
GKHLDSFADLLNFSITPALVILILDQSLSATLAAVTLVFTAILRLSLFGMPGTSAGGGYQ
GLPTTYSGFVFALALMGLSAGHYGAGVIVGLMALVGILQVTSLRIPKYGAPSTVACFAML
FGLSTIYFHPW